Brandão-Neto Daniel de Oliveira, Mello João Victor Zebende, Marceliano-Alves Marilia Fagury Videira, Carvalho Coutinho Thais Machado de, Marceliano Eduardo Fagury Videira, Galhardi Michelle Paiva Weydt, Tavares Vívian Souza, Muzy Dias Aline Peçanha, Lins Renata Ximenes
Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
School of Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
Eur J Dent. 2021 Jul;15(3):533-538. doi: 10.1055/s-0041-1723068. Epub 2021 Feb 3.
The aim of present study was to assess the cytotoxicity and antimicrobial efficacy of 2% peracetic acid (PAA) compared with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX).
For the cytotoxicity test, 100 µl of the tested solutions were added in 12 wells with ECV 304 endothelial cells in each group: NaOCl, CHX, and PAA, in addition to the control group. Each solution was evaluated after 24 hours of contact in four dilutions: 0.2, 0.1, 0.05 and 0.025 through mitochondrial function using MTT colorimetric assay. In the antimicrobial evaluation, 40 dentin blocks 5 mm in length and 0.2 g in weight were incubated with 400 µl of suspension for 21 days at 37°C. The contaminated samples were divided into three experimental groups within 5 minutes of contact: NaOCl group, CHX group, PAA group, as well as the positive control group. The specimens received treatment and were transferred to a tube with saline for serial dilution of the solution and seeding for isolation and colony forming unit (CFU) count.
The results obtained were expressed as mean (A570 nm) ± standard deviation (SD) and in a multiple linear regression model and multiple comparisons conducted.
The antimicrobial evaluation revealed that the NaOCl and CHX groups showed a statistically significant difference compared with the control group ( < 0.001), while the PAA reduced only the CFU growth. It can be concluded that, among the agents tested, PAA expressed greater cell viability, followed by CHX and NaOCl. However, it did not show greater antimicrobial activity in the mature biofilm of
本研究旨在评估2%过氧乙酸（PAA）与5.25%次氯酸钠（NaOCl）和2%氯己定（CHX）相比的细胞毒性和抗菌效果。
进行细胞毒性试验时，在每组含有ECV 304内皮细胞的12孔板中加入100 μl受试溶液：NaOCl组、CHX组、PAA组，此外还有对照组。通过MTT比色法利用线粒体功能在4种稀释度（0.2、0.1、0.05和0.025）下接触24小时后对每种溶液进行评估。在抗菌评估中，将40个长度为5 mm、重量为0.2 g的牙本质块与400 μl悬浮液在37°C下孵育21天。在接触5分钟内将受污染的样本分为三个实验组：NaOCl组、CHX组、PAA组，以及阳性对照组。对标本进行处理，然后转移到装有盐水的试管中对溶液进行系列稀释并接种以进行分离和菌落形成单位（CFU）计数。
所得结果以平均值（A570 nm）±标准差（SD）表示，并进行多元线性回归模型和多重比较。
抗菌评估显示，与对照组相比，NaOCl组和CHX组有统计学显著差异（<0.001），而PAA仅降低了CFU生长。可以得出结论，在所测试的药剂中，PAA表现出更高的细胞活力，其次是CHX和NaOCl。然而，它在成熟生物膜中并未表现出更强的抗菌活性。
