Winkles J, Lunec J, Deverill I
Clin Chem. 1987 May;33(5):685-9.
This is an improved assay of C-reactive protein in serum, for use with the Baker "Encore" centrifugal analyzer. Features of this assay include: 250-specimen throughput per hour, within-batch CV 2.2%, between-batch CV 2.7%, no antigen-excess problems up to 1000 mg/L, negligible interference from rheumatoid factor, and good correlation (r = 0.99) with radial immunodiffusion. The method is inexpensive and automated, involving no predilution steps. It can be adapted for use in a wide range of systems and can be used for single urgent estimations.
这是一种用于贝克“安可”离心分析仪的血清C反应蛋白改进检测方法。该检测方法的特点包括:每小时可处理250个样本,批内变异系数为2.2%,批间变异系数为2.7%,在高达1000mg/L时无抗原过剩问题,类风湿因子干扰可忽略不计,与放射免疫扩散法具有良好的相关性(r = 0.99)。该方法成本低廉且自动化,无需预稀释步骤。它可适用于多种系统,也可用于单个紧急检测。