COX-2、NF-κB 及血常规指标在恶性腹膜间皮瘤预后评估中的价值。

The Value of COX-2, NF-κB, and Blood Routine Indexes in the Prognosis of Malignant Peritoneal Mesothelioma.

机构信息

Department of Gastroenterology, Cangzhou Central Hospital, Cangzhou, China.

Department of Gastroenterology, Cangzhou Central Hospital, Cangzhou, China,

出版信息

Oncol Res Treat. 2019;42(6):334-341. doi: 10.1159/000499677. Epub 2019 May 7.

DOI:10.1159/000499677

PMID:31063993

Abstract

BACKGROUND/AIMS: To investigate differences in blood routine indexes and the expression of cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF-κB) in malignant peritoneal mesothelioma (MPeM) and their relationship with clinical prognosis.

METHODS

We investigated changes in blood routine indexes between the MPeM patients and healthy subjects and detected the expression of COX-2 and NF-κB in peritoneal tissues by a streptavidin-peroxidase immunohistochemistry method. Potential prognostic factors were analyzed including age, gender, white blood cell count (WBC), absolute neutrophil count (ANC), absolute lymphocyte count (ALC), absolute platelet count (APC), absolute monocyte count (AMC), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), COX-2, and NF-κB. Cox regression model analysis established independent factors for the survival prognosis of the patients.

RESULTS

Compared with the control group, AMC, MXD%, ANC, neutrophilic granulocyte percentage (NEUT%), APC, NLR, MLR, and PLR were markedly increased (p < 0.05) in the MPeM group. The positivity rates for COX-2 and NF-κB expression were 59.4 and 44.9%, respectively. Single factor analyses indicated that PLR, NLR, MLR, COX-2, and NF-κB were factors that affected the overall survival of MPeM patients, but multivariate analyses identified MLR and COX-2 as independent prognostic factors.

CONCLUSIONS

High blood levels of MLR and COX-2 are adverse prognostic factors for patients with MPeM.

摘要

背景/目的：研究恶性腹膜间皮瘤（MPeM）患者血液常规指标和环氧化酶-2（COX-2）及核因子-κB（NF-κB）表达的差异及其与临床预后的关系。

方法

通过酶联免疫吸附法检测 MPeM 患者与健康受试者血液常规指标的变化，并采用链霉亲和素-过氧化物酶免疫组化法检测腹膜组织中 COX-2 和 NF-κB 的表达。分析年龄、性别、白细胞计数（WBC）、中性粒细胞绝对值（ANC）、淋巴细胞绝对值（ALC）、血小板绝对值（APC）、单核细胞绝对值（AMC）、中性粒细胞与淋巴细胞比值（NLR）、血小板与淋巴细胞比值（PLR）、单核细胞与淋巴细胞比值（MLR）、COX-2 和 NF-κB 等可能的预后因素。Cox 回归模型分析确立了患者生存预后的独立因素。

结果

与对照组相比，MPeM 组的 AMC、MXD%、ANC、中性粒细胞百分比（NEUT%）、APC、NLR、MLR 和 PLR 显著升高（p<0.05）。COX-2 和 NF-κB 表达的阳性率分别为 59.4%和 44.9%。单因素分析表明，PLR、NLR、MLR、COX-2 和 NF-κB 是影响 MPeM 患者总生存的因素，但多因素分析表明 MLR 和 COX-2 是独立的预后因素。