Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Institute of Feed Science, College of Animal Science, Zhejiang University, 310058 Hangzhou, China.
Poult Sci. 2019 Oct 1;98(10):5028-5039. doi: 10.3382/ps/pez226.
This study aimed to investigate the effects of Lactobacillus plantarum 16 (Lac16) and Paenibacillus polymyxa 10 (BSC10) on intestinal barrier function, antioxidative capacity, apoptosis, immune response, and biochemical parameters in broilers. A total of 540 one-day-old broiler chicks (Cobb500) were randomly allocated to three groups of 180 birds, and fed either a basal diet or a basal diet supplemented with 108 colony-forming units Lac16 or BSC10 per kilogram feed for 21 D. The results revealed that both Lac16 and BSC10 maintained ileal mucosal morphology, and BSC10 regulated the expression of barrier function-related genes. Birds fed with probiotics decreased malondialdehyde level in jejunal mucosa and serum, and the increased activities of hepatic GSH-Px and jejunal CAT were observed in BSC10 group (P < 0.05). Immunohistochemistry of Bax, Bcl-2 and proliferating cell nuclear antigen and TUNEL-immunofluorescence assay demonstrated that Lac16 and BSC10 exerted beneficial effects on cell apoptosis and proliferation, as indicated by the gene expression of down-regulated Bax and p53 as well as a significant upregulation of Bcl-2 (P < 0.05). In addition, Lac16 and BSC10 significantly increased NO production and iNOS activity in liver and jejunal mucosa, and gene expression of IFN-γ (P < 0.01), IL-6 (P < 0.05), and IL-10 (P < 0.05 and P < 0.01, respectively) in ileum mucosa, whereas markedly decreased the expression of Cox2 (P < 0.05). Furthermore, it was found that Lac16 and BSC10 significantly reduced levels of alkaline phosphatase (P < 0.05 and P < 0.01, respectively) and creatine kinase (P < 0.05). Moreover, BSC10 significantly reduced uric acid (P < 0.05) and low-density lipoprotein levels (P < 0.01). Taken together, Lac16 and BSC10 could improve intestinal and body health status of broilers by increasing intestinal barrier function, anti-oxidative capacity and immunity, and decreasing cell apoptosis with strain-specificity.
本研究旨在探讨植物乳杆菌 16（Lac16）和多粘类芽孢杆菌 10（BSC10）对肉鸡肠道屏障功能、抗氧化能力、细胞凋亡、免疫反应和生化参数的影响。将 540 只 1 日龄肉鸡（Cobb500）随机分为 3 组，每组 180 只，分别饲喂基础日粮或基础日粮添加 108 菌落形成单位 Lac16 或 BSC10/kg 饲料，试验期 21d。结果表明，Lac16 和 BSC10 均维持了回肠黏膜形态，BSC10 调节了屏障功能相关基因的表达。添加益生菌可降低十二指肠黏膜和血清中丙二醛水平，BSC10 组肝 GSH-Px 和空肠 CAT 活性增加（P<0.05）。Bax、Bcl-2 和增殖细胞核抗原的免疫组化和 TUNEL-免疫荧光检测表明，Lac16 和 BSC10 对细胞凋亡和增殖具有有益作用，Bax 和 p53 基因表达下调，Bcl-2 显著上调（P<0.05）。此外，Lac16 和 BSC10 显著增加了肝脏和空肠黏膜中 NO 的产生和 iNOS 的活性，以及回肠黏膜中 IFN-γ（P<0.01）、IL-6（P<0.05）和 IL-10（P<0.05 和 P<0.01）的基因表达，而 Cox2 的表达显著降低（P<0.05）。此外，还发现 Lac16 和 BSC10 显著降低了碱性磷酸酶（P<0.05 和 P<0.01）和肌酸激酶（P<0.05）的水平。此外，BSC10 显著降低了尿酸（P<0.05）和低密度脂蛋白的水平（P<0.01）。综上所述，Lac16 和 BSC10 可通过提高肠道屏障功能、抗氧化能力和免疫力，降低细胞凋亡来改善肉鸡的肠道和全身健康状况，且具有菌株特异性。
