Poult Sci. 2017 Aug 1;96(8):2576-2586. doi: 10.3382/ps/pex061.
Two studies were performed to assess the efficacy of Lactobacillus plantarum B1 in prevention of pathogenic Escherichia coli K88 gastrointestinal infection in broilers. In an in vitro study, L. plantarum B1 showed resistance to acid and bile and inhibited the growth of E. coli K88. Additionally, L. plantarum B1 exhibited high ability to adhere to broiler embryo ileal epithelium. In an animal trial, 240 broilers at 1 d of age were randomly assigned to one of 4 treatment arms: negative control (NC) broilers fed a basal diet and not challenged; positive control (PC) broilers fed a basal diet and challenged with E. coli K88; L. plantarum (LP) treatment broilers fed a basal diet containing 2 × 109 cfu/kg L. plantarum B1 and challenged with E. coli K88; and antibiotic treatment (Anti) broilers fed a basal diet supplemented with colistin sulfate (20 mg/kg) and challenged with E. coli K88. Broilers fed L. plantarum B1 had greater (P ≤ 0.05) BW than those in the PC treatment on d 14 and 28. Dietary L. plantarum B1 decreased (P < 0.05) E. coli counts in the cecal contents on d 10 and 14, and increased (P < 0.05) cecal lactic acid bacteria (LAB) on d 8, 10, 14, and 28 compared with the PC treatment. Dietary supplementation of L. plantarum B1 increased (P < 0.05) the ileal mucosal secretory IgA concentration and reduced (P < 0.05) IL-2, IL-4, IFN-γ, and tumor necrosis factor-α levels in the ileum. Overall, these results suggest dietary supplementation of L. plantarum B1 promotes growth performance, lowers cecal E. coli counts, and increases the population of cecal LAB, as well as improves intestinal mucosal immunity in E. coli K88-challenged broilers.
两项研究评估了植物乳杆菌 B1 预防肉鸡致病性大肠杆菌 K88 胃肠道感染的功效。在体外研究中，植物乳杆菌 B1 表现出对酸和胆汁的抗性，并抑制大肠杆菌 K88 的生长。此外，植物乳杆菌 B1 对肉鸡胚回肠上皮的黏附能力很高。在动物试验中，240 只 1 日龄肉鸡被随机分为 4 个处理组之一：阴性对照组（NC）肉鸡饲喂基础日粮且不攻毒；阳性对照组（PC）肉鸡饲喂基础日粮且攻毒大肠杆菌 K88；植物乳杆菌（LP）处理组肉鸡饲喂含 2×109cfu/kg 植物乳杆菌 B1 的基础日粮且攻毒大肠杆菌 K88；抗生素处理组（Anti）肉鸡饲喂添加硫酸粘菌素（20mg/kg）的基础日粮且攻毒大肠杆菌 K88。饲喂植物乳杆菌 B1 的肉鸡在第 14 和 28 天的 BW 显著（P≤0.05）高于 PC 组。日粮添加植物乳杆菌 B1 降低（P<0.05）第 10 和 14 天盲肠内容物中大肠杆菌的数量，增加（P<0.05）第 8、10、14 和 28 天盲肠乳酸菌（LAB）的数量，与 PC 组相比。日粮添加植物乳杆菌 B1 增加（P<0.05）回肠黏膜分泌型 IgA 浓度，降低（P<0.05）回肠中 IL-2、IL-4、IFN-γ 和肿瘤坏死因子-α的水平。总的来说，这些结果表明，日粮添加植物乳杆菌 B1 可促进生长性能，降低盲肠大肠杆菌数量，增加盲肠 LAB 数量，并改善大肠杆菌 K88 攻毒肉鸡的肠道黏膜免疫。
