Dubreuil Matthieu, Tissier Florine, Le Roy Lucas, Pennec Jean-Pierre, Rivet Sylvain, Giroux-Metges Marie-Agnès, Le Grand Yann
Université de Bretagne Occidentale, Laboratoire d'optique et de magnétisme OPTIMAG EA 938, IBSAM, 6 avenue Le Gorgeu, Brest, 29238, France.
Université de Bretagne Occidentale, Laboratoire optimisation des régulations physiologiques ORPHY EA 4324, IBSAM, 6 avenue Le Gorgeu, Brest, 29238, France.
Biomed Opt Express. 2018 Nov 19;9(12):6350-6358. doi: 10.1364/BOE.9.006350. eCollection 2018 Dec 1.
Polarization-resolved second harmonic generation (P-SHG) microscopy is able to probe the sub-micrometer structural organization of myosin filaments within skeletal muscle. In this study, P-SHG microscopy was used to analyze the structural consequences of sepsis, which is the main cause of the critical illness polyneuromyopathy (CIPNM). Experiments conducted on two populations of rats demonstrated a significant difference of the anisotropy parameter between healthy and septic groups, indicating that P-SHG microscopy is promising for the diagnosis of CIPNM. The difference, which can be attributed to a change of myosin conformation at the sub-sarcomere scale, cannot be evidenced by classical SHG imaging.
偏振分辨二次谐波产生（P-SHG）显微镜能够探测骨骼肌中肌球蛋白丝的亚微米结构组织。在本研究中，P-SHG显微镜被用于分析脓毒症的结构后果，脓毒症是危重病性多神经病性肌病（CIPNM）的主要病因。在两组大鼠上进行的实验表明，健康组和脓毒症组之间的各向异性参数存在显著差异，这表明P-SHG显微镜在CIPNM的诊断方面具有前景。这种差异可归因于肌小节亚尺度上肌球蛋白构象的变化，而传统的SHG成像无法证实这一点。
