Fu Songzhe, Hao Jingwei, Jin Shibo, Wu Kui, Wang Yi, Ye Shigen, Liu Ying, Li Ruijun
College of Marine Technology and Environment, Dalian Ocean University, Dalian, China.
College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
Front Public Health. 2019 Apr 24;7:100. doi: 10.3389/fpubh.2019.00100. eCollection 2019.
Non-O1/O139 is increasingly reported in the clinical settings. However, intestinal infections via the consumption of non-O1/O139 -carrying seafood are rarely documented in China. In this study, we reported a case of mild watery diarrhea in a young male, caused by non-O1/O139 in the downstream of Liaohe River. Epidemiological investigation showed that this intestinal infection potentially associated with the raw consumption of mollusc. Prior to this finding, we conducted a 6-month pathogen surveillance of three locations along the Liaohe River and identified three environmental non-O1/O139 strains. To confirm the epidemiological links between clinical and environmental strains, high-resolution genomic typing was employed and revealed that isolated from human stool sample was genomically related to the one found in local mollusc and shared a common ancestor with other environmental strains obtained in the upstream sites of the Liaohe River. This fact suggests that the river is a natural reservoir for non-O1/O139 which poses a potential threat to the public health. In summary, our results deepened the insights on the transmission of non-pandemic strains and underscored the significance of genomic surveillance for drinking water along the river sites.
非O1/O139在临床环境中的报告日益增多。然而，在中国，通过食用携带非O1/O139的海鲜引起的肠道感染鲜有记录。在本研究中，我们报告了一例辽河下游一名年轻男性因非O1/O139引起的轻度水样腹泻病例。流行病学调查显示，这种肠道感染可能与生食软体动物有关。在此发现之前，我们对辽河沿线三个地点进行了为期6个月的病原体监测，鉴定出三株环境非O1/O139菌株。为了确认临床菌株与环境菌株之间的流行病学联系，采用了高分辨率基因组分型，结果显示从人类粪便样本中分离出的菌株在基因组上与当地软体动物中发现的菌株相关，并与在辽河上游地点获得的其他环境菌株有共同的祖先。这一事实表明，该河流是非O1/O139的天然宿主，对公众健康构成潜在威胁。总之，我们的结果加深了对非大流行菌株传播的认识，并强调了对沿河地点饮用水进行基因组监测的重要性。
