Ahmed Ibrahim, Huebner Holger, Mamoori Yaseen Ismael, Buchholz Rainer
Department of Chemical and Biological Engineering, Bioprocess Engineering Institute, Friedrich Alexander University, Erlangen-Nuremberg, Erlangen, Germany.
College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
Cytotechnology. 2019 Jun;71(3):723-731. doi: 10.1007/s10616-019-00317-2. Epub 2019 May 8.
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Spodoptera littoralis multiple nucleopolyhedrovirus (SpliMNPV) belong to group I and group II nucleopolyhedroviruses, respectively and can replicate in a wide range of insect species. In this study, the ability of newly established S. littoralis cell lines to support replication of AcMNPV and SpliMNPV was examined. The microscopic observations showed that the S. littoralis cells infected with AcMNPV exhibited morphological changes such as cells breaking into small bodies and forming apoptosis-like bodies post-infection. Nuclear DNA fragmentation was observed in all AcMNPV-infected cell lines through DNA gel electrophoresis analysis. Therefore, the virus replication was unsuccessful in most of cells, which were able to abort the virus replication. On the other hand, cells that were infected with SpliMNPV did not show similar morphological changes and no small bodies were formed. In addition, SpliMNPV succeeded to infect the cells, replicate, and form viral occlusion bodies inside the infected cells. In suspension culture, S. littoralis cells, which were infected with AcMNPV, accumulated as composed balls in shaker flasks after infection overnight, with cell density decreasing dramatically. In contrast, there was no cell clumping seen in the infected cells with SpliMNPV and the uninfected cells. In conclusion, the newly established embryonic S. littoralis cells were highly susceptible to SpliMNPV, whereas the cells were non-permissive to AcMNPV, yet they still underwent programmed cell death.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）和滨海斜纹夜蛾多核衣壳核型多角体病毒（SpliMNPV）分别属于I组和II组核型多角体病毒，可在多种昆虫物种中复制。在本研究中，检测了新建立的滨海斜纹夜蛾细胞系支持AcMNPV和SpliMNPV复制的能力。显微镜观察显示，感染AcMNPV的滨海斜纹夜蛾细胞表现出形态变化，如感染后细胞裂解成小体并形成凋亡样小体。通过DNA凝胶电泳分析在所有感染AcMNPV的细胞系中均观察到核DNA片段化。因此，病毒在大多数细胞中复制未成功，这些细胞能够中止病毒复制。另一方面，感染SpliMNPV的细胞未显示出类似的形态变化，也未形成小体。此外，SpliMNPV成功感染细胞、复制并在感染细胞内形成病毒包涵体。在悬浮培养中，感染AcMNPV的滨海斜纹夜蛾细胞在过夜感染后在摇瓶中聚集成复合球，细胞密度急剧下降。相比之下，感染SpliMNPV的细胞和未感染的细胞中均未观察到细胞聚集。总之，新建立的滨海斜纹夜蛾胚胎细胞对SpliMNPV高度敏感，而对AcMNPV不敏感，但它们仍会经历程序性细胞死亡。
