Orthopedic Department, Second Affiliated Hospital of Harbin Medical University, No. 148 Baojian Road, Nangang District, Harbin, Heilongjiang, China.
Surgical Emergency, First Affiliated Hospital of Harbin Medical University, Harbin, China.
J Orthop Surg Res. 2021 Jan 19;16(1):68. doi: 10.1186/s13018-021-02216-9.
Osteoarthritis (OA) is a leading cause of disability. The incidence of OA is progressively rising due to the diminishing levels of physical activity and ever-expanding aging population. However, the mainstay for OA treatment only can improve symptoms without delay the progression of this severe disease. This study aimed to explore the biological role and clinical function of lncRNA HAND2-AS1 in OA.
Blood samples and synovial fluid were collected from OA patients and normal subjects. HAND2-AS1 expression was detected by qRT-PCR and IL-6 expression was detected by ELISA. The plasma levels of HAND2-AS1 were also detected in different ages, stages, and gender of OA patients and controls. Furthermore, the ROC curve was used to analyze whether HAND2-AS1 can distinguish OA patients from normal subjects. Also, Pearson correlation coefficient analysis was used to analyze the correlation between lncRNA HAND2-AS1 and IL-6. In addition, Western blot was used to detect the IL-6 level upon HAND2-AS1 over-expression in chondrocytes and qRT-PCR was used to detect the HAND2-AS1 level after endogenous IL-6 treatment.
HAND2-AS1 and IL-6 were dysregulated in plasma and synovial fluid of OA patients. The expression of HAND2-AS1 in plasma of OA patients was decreased with aging and progression. Furthermore, HAND2-AS1 downregulation effectively distinguished OA patients from the healthy controls. Over-expression of HAND2-AS1 inhibited IL-6 expression in chondrocytes, while treatment with exogenous IL-6 did not affect HAND2-AS1 expression.
HAND2-AS1 effectively distinguished OA patients from the healthy controls and regulates IL-6 expression in human chondrocytes.
ChiCTR, ChiCTR2000038635 . Registered 11 February 2019.
骨关节炎（OA）是导致残疾的主要原因。由于体力活动水平下降和不断扩大的老龄化人口，OA 的发病率正在逐渐上升。然而，OA 治疗的主要方法只能改善症状，而不能延缓这种严重疾病的进展。本研究旨在探讨长链非编码 RNA HAND2-AS1 在 OA 中的生物学作用和临床功能。
收集 OA 患者和正常对照者的血液样本和滑膜液。通过 qRT-PCR 检测 HAND2-AS1 的表达，通过 ELISA 检测 IL-6 的表达。还检测了不同年龄、分期和性别的 OA 患者和对照者的血浆 HAND2-AS1 水平。此外，使用 ROC 曲线分析 HAND2-AS1 是否可以区分 OA 患者和正常对照者。还使用 Pearson 相关系数分析分析长链非编码 RNA HAND2-AS1 与 IL-6 之间的相关性。另外，通过 Western blot 检测 HAND2-AS1 过表达对软骨细胞中 IL-6 水平的影响，通过 qRT-PCR 检测内源性 IL-6 处理后 HAND2-AS1 水平。
HAND2-AS1 和 IL-6 在 OA 患者的血浆和滑膜液中失调。OA 患者血浆中 HAND2-AS1 的表达随着年龄的增长和疾病的进展而降低。此外，HAND2-AS1 下调可有效区分 OA 患者和健康对照者。HAND2-AS1 过表达抑制软骨细胞中 IL-6 的表达，而外源性 IL-6 处理对 HAND2-AS1 表达没有影响。
HAND2-AS1 可有效区分 OA 患者和健康对照者，并调节人软骨细胞中 IL-6 的表达。
ChiCTR，ChiCTR2000038635。注册于 2019 年 2 月 11 日。
