Department of Thyroid Breast Surgery, Cangzhou Central Hospital, Cangzhou City, People's Republic of China.
Cancer Biother Radiopharm. 2019 May;34(4):258-263. doi: 10.1089/cbr.2018.2684.
Downregulation of LncRNA LINC-PINT has been observed in different types of cancer cells, indicating its role as a tumor suppressor. Expression of LINC-PINT and miRNA-21 in tumor tissues and adjacent healthy tissues of 56 patients with osteosarcoma was detected by real-time quantitative PCR. Correlations between expression levels of LncRNA LINC-PINT and miRNA-21 were analyzed by Pearson's correlation coefficient. In this study, we found that LncRNA LINC-PINT was inhibited, whereas miRNA-21 was promoted in tumor tissues than in adjacent healthy tissues of patients with osteosarcoma. Expression levels of LncRNA LINC-PINT were affected by both tumor size and tumor metastasis. LncRNA LINC-PINT and miRNA-21 were significantly and reversely correlated in both tumor cells and adjacent healthy tissues. LncRNA LINC-PINT overexpression led to downregulated miRNA-21 expression in cancer cells, whereas miRNA-21 overexpression did not significantly affect LINC-PINT expression. Overexpression of LncRNA LINC-PINT inhibited whereas miRNA-21 overexpression promoted cancer cell proliferation, migration, and invasion. In addition, miRNA-21 overexpression partially rescued the inhibited cell proliferation, migration, and invasion mediated by LncRNA LINC-PINT overexpression. Therefore, LncRNA LINC-PINT may inhibit cancer cell proliferation, invasion, and migration in osteosarcoma by downregulating miRNA-21.
LncRNA LINC-PINT 的表达下调已在不同类型的癌细胞中观察到，表明其作为肿瘤抑制因子的作用。通过实时定量 PCR 检测了 56 例骨肉瘤患者肿瘤组织和相邻正常组织中 LINC-PINT 和 miRNA-21 的表达。通过 Pearson 相关系数分析 LncRNA LINC-PINT 和 miRNA-21 表达水平之间的相关性。 在这项研究中，我们发现 LncRNA LINC-PINT 在骨肉瘤患者的肿瘤组织中受到抑制，而 miRNA-21 则在肿瘤组织中受到促进。LncRNA LINC-PINT 的表达水平受到肿瘤大小和肿瘤转移的影响。LncRNA LINC-PINT 和 miRNA-21 在肿瘤细胞和相邻正常组织中均呈显著负相关。LncRNA LINC-PINT 的过表达导致癌细胞中 miRNA-21 的表达下调，而 miRNA-21 的过表达对 LINC-PINT 的表达没有显著影响。LncRNA LINC-PINT 的过表达抑制而 miRNA-21 的过表达促进癌细胞的增殖、迁移和侵袭。此外，miRNA-21 的过表达部分挽救了 LncRNA LINC-PINT 过表达介导的抑制细胞增殖、迁移和侵袭。 因此，LncRNA LINC-PINT 可能通过下调 miRNA-21 抑制骨肉瘤中癌细胞的增殖、侵袭和迁移。
