Department of Thoracic surgery, Sichuan Cancer Hospital, Chengdu City, P.R. China.
Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, P.R. China.
Cancer Biother Radiopharm. 2021 Apr;36(3):273-279. doi: 10.1089/cbr.2019.3167. Epub 2020 May 13.
LncRNA long intergenic non-protein coding RNA p53 induced transcript (LINC-PINT) is downregulated in multiple types of cancer cells. The authors explored the possible involvement of LINC-PINT in esophageal squamous cell carcinoma (ESCC). Sixty-two patients with early-stage ESCC were included in this study. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect gene expression in plasma from ESCC patients and healthy controls. Diagnostic analysis was performed by receiver operating characteristic (ROC) curve. Transfections were performed to analyze gene interactions. Cell invasion and migration were analyzed by Transwell assays. Plasma LINC-PINT was downregulated and microRNA (miRNA)-21 was upregulated in early-stage ESCC patients. Diagnostic analysis by ROC curve revealed that downregulation of lncRNA LINC-PINT distinguished ESCC patients from healthy controls. Plasma levels of LINC-PINT and miRNA-21 were negatively correlated in ESCC patients. After surgical resection, only local recurrence was observed during 3-years of follow-up. LINC-PINT expression was further downregulated in recurrent patients but not in nonrecurrent patients. ROC curve analysis revealed that plasma levels of LINC-PINT at 12 months before recurrence can be used to distinguish ESCC patients from healthy controls. Overexpression of LINC-PINT could inhibit the expression of miRNA-21 in human ESCC cells, whereas LINC-PINT expression was not altered by miR-21 overexpression. Therefore, downregulation of LINC-PINT participated in the recurrence of ESCC possibly by interacting with miRNA-21.
长链非编码 RNA p53 诱导转录物（LINC-PINT）在多种癌细胞中下调。作者探讨了 LINC-PINT 可能参与食管鳞状细胞癌（ESCC）的情况。本研究纳入了 62 例早期 ESCC 患者。采用逆转录-聚合酶链反应（RT-PCR）检测 ESCC 患者和健康对照者血浆中的基因表达。通过接收者操作特征（ROC）曲线进行诊断分析。转染用于分析基因相互作用。通过 Transwell 分析检测细胞侵袭和迁移。早期 ESCC 患者血浆 LINC-PINT 下调，miRNA-21 上调。ROC 曲线分析显示，lncRNA LINC-PINT 的下调可将 ESCC 患者与健康对照者区分开来。ESCC 患者血浆 LINC-PINT 和 miRNA-21 水平呈负相关。手术后 3 年随访中仅观察到局部复发。复发患者的 LINC-PINT 表达进一步下调，但非复发患者无此变化。ROC 曲线分析显示，复发前 12 个月血浆 LINC-PINT 水平可用于区分 ESCC 患者和健康对照者。LINC-PINT 在人 ESCC 细胞中的过表达可抑制 miRNA-21 的表达，而 miR-21 的过表达不改变 LINC-PINT 的表达。因此，LINC-PINT 的下调可能通过与 miRNA-21 相互作用参与 ESCC 的复发。
