a Department of Pharmaceutical Technology , University Faculty of Pharmacy , Kayseri , Turkey.
b Department of Pharmacognosy Erciyes , University Faculty of Pharmacy , Kayseri , Turkey.
J Microencapsul. 2019 Mar;36(2):180-191. doi: 10.1080/02652048.2019.1617363. Epub 2019 May 27.
The study was aimed to evaluate the effectiveness of rosmarinic acid (RA) loaded ethosomes (ETHs) and liposomes (LPs) when subjected to the transdermal application. RA-loaded ETHs and LPs were prepared, optimised, and characterised. The permeation studies of formulations using mouse abdominal skin were performed. Antioxidant activities and the inhibitory effects of formulations on collagenase and elastase enzymes were measured. Optimised ethosomal formulation (F3) was showed nanometric size range (138 ± 1.11 nm) and greatest entrapment (55 ± 1.80%), was selected for further transdermal permeation studies. Skin permeation profile of the nanoformulations analysed by HPLC revealed an enhanced permeation of ETHs. Transdermal flux of ETHs was found to be higher than RA solution and LPs. Enzyme inhibitions of ETHs were the significant difference found between ETHs and LPs ( < 0.05). ETHs were found to be more effective and successful than LPs. Results suggest that ETHs are more effective than LPs for transdermal delivery of RA.
本研究旨在评估迷迭香酸（RA）负载的醇质体（ETHs）和脂质体（LPs）经皮给药的效果。制备、优化并表征了负载 RA 的 ETHs 和 LPs。使用小鼠腹部皮肤进行了制剂的渗透研究。测量了制剂的抗氧化活性以及对胶原酶和弹性蛋白酶的抑制作用。优化的醇质体制剂（F3）显示出纳米尺寸范围（138±1.11nm）和最大包封率（55±1.80%），被选择用于进一步的经皮渗透研究。通过 HPLC 分析纳米制剂的皮肤渗透谱显示出 ETHs 的增强渗透。发现 ETHs 的透皮通量高于 RA 溶液和 LPs。ETHs 的酶抑制作用与 LPs 之间存在显著差异（<0.05）。发现 ETHs 比 LPs 更有效。结果表明，ETHs 比 LPs 更适合 RA 的经皮传递。
