Hendricks Sarah A, Vella Chantal A, New Daniel D, Aunjum Afiya, Antush Maximilian, Geidl Rayme, Andrews Kimberly R, Balemba Onesmo B
Institute for Interdisciplinary Data Sciences, University of Idaho, Moscow, ID 83843, USA.
Department of Movement Sciences, University of Idaho, Moscow, ID 83843, USA.
Microorganisms. 2023 Mar 15;11(3):758. doi: 10.3390/microorganisms11030758.
Alterations in the composition of the gut microbiota is thought to play a key role in causing type 2 diabetes, yet is not fully understood, especially at the strain level. Here, we used long-read DNA sequencing technology of 16S-ITS-23S rRNA genes for high-resolution characterization of gut microbiota in the development of type 2 diabetes. Gut microbiota composition was characterized from fecal DNA from 47 participants divided into 4 cohorts based on glycemic control: normal glycemic control (healthy; = 21), reversed prediabetes (prediabetes/healthy; = 8), prediabetes ( = 8), or type 2 diabetes ( = 10). A total of 46 taxa were found to be possibly related to progression from healthy state to type 2 diabetes. DSM 18228, DSM 20438, and ATCC 15703 could confer resistance to glucose intolerance. On the other hand, YIT 12061 may be pathogenic as it was found to be more abundant in type 2 diabetes participants than other cohorts. This research increases our understanding of the structural modulation of gut microbiota in the pathogenesis of type 2 diabetes and highlights gut microbiota strains, with the potential for targeted opportunistic pathogen control or consideration for probiotic prophylaxis and treatment.
肠道微生物群组成的改变被认为在2型糖尿病的发病中起关键作用，但尚未完全明确，尤其是在菌株水平上。在此，我们使用16S-ITS-23S rRNA基因的长读长DNA测序技术对2型糖尿病发展过程中的肠道微生物群进行高分辨率表征。根据血糖控制情况，将47名参与者分为4个队列，从其粪便DNA中表征肠道微生物群组成：血糖正常控制（健康；n = 21）、逆转的糖尿病前期（糖尿病前期/健康；n = 8）、糖尿病前期（n = 8）或2型糖尿病（n = 10）。共发现46个分类单元可能与从健康状态发展到2型糖尿病有关。DSM 18228、DSM 20438和ATCC 15703可赋予对葡萄糖不耐受的抗性。另一方面，YIT 12061可能具有致病性，因为在2型糖尿病参与者中发现其比其他队列更为丰富。这项研究增进了我们对2型糖尿病发病机制中肠道微生物群结构调节的理解，并突出了肠道微生物群菌株，具有针对性地控制机会性病原体或考虑益生菌预防和治疗的潜力。
