Naparstek E, Wagner K, Harrison D, Greenberger J S
Acta Haematol. 1987;77(1):1-5. doi: 10.1159/000205939.
A method for continuous intravenous infusion of unanesthetized adult C3H/HeJ or Wx/Wv anemic mice was designed using cage immobilization and a tail vein catheter connected to a model 940 Harvard infusion pump. Infusates included: WEHI-3 cell line dialyzed, 5 times concentrated conditioned medium containing multi-colony-stimulating factor (C-SF) interleukin 3 (IL-3); purified murine IL-3; bacterial endotoxin; serum-free medium, or normal saline. Mice were monitored at days 5-7 after infusion for complete peripheral blood counts and production of granulocytes in vitro by explanted marrow in long-term bone marrow cultures. We observed a stimulatory effect of WEHI-3 conditioned medium infusion that was not attributable to endotoxin and produced significant increases in peripheral blood WBC count and neutrophils, colony-forming units in spleen and numbers of granulocyte/macrophage colony-forming unit culture responsive to both C-SF-1 (L cell C-SF) and multi-C-SF in vitro. This infusion method should prove valuable for test of the in vivo effects of purified growth factors and molecularly cloned hematopoietins.
设计了一种用于连续静脉输注未麻醉的成年C3H/HeJ或Wx/Wv贫血小鼠的方法，该方法使用笼子固定以及连接到940型哈佛输液泵的尾静脉导管。输注液包括：经透析的WEHI-3细胞系、含有多集落刺激因子（C-SF）白细胞介素3（IL-3）的5倍浓缩条件培养基；纯化的小鼠IL-3；细菌内毒素；无血清培养基或生理盐水。在输注后第5至7天监测小鼠的全外周血细胞计数以及长期骨髓培养中体外培养的骨髓产生粒细胞的情况。我们观察到WEHI-3条件培养基输注具有刺激作用，该作用并非由内毒素引起，并且可使外周血白细胞计数和中性粒细胞显著增加，脾脏中的集落形成单位以及体外对C-SF-1（L细胞C-SF）和多集落刺激因子均有反应的粒细胞/巨噬细胞集落形成单位培养物数量显著增加。这种输注方法对于测试纯化生长因子和分子克隆造血因子的体内效应应具有重要价值。
