Hernandez D, Rowe J J
Biology Department, University of Dayton, Ohio 45469.
J Biol Chem. 1988 Jun 15;263(17):7937-9.
It has recently been demonstrated that oxygen inhibits nitrate uptake by denitrifying Pseudomonas aeruginosa. The purpose of the present investigation was to determine if this novel mechanism of regulation is universal for the regulation of nitrate respiration in other widely divergent species of bacteria. Nitrate transport by whole cell suspensions was completely and reversibly inhibited in 11 out of 12 species tested, whereas nitrate reduction by cell-free extracts was not affected by oxygen or was only partially inhibited in some cases. These results indicate that oxygen inhibition of nitrate uptake is a general regulatory phenomenon.
最近有研究表明，氧气会抑制反硝化铜绿假单胞菌对硝酸盐的摄取。本研究的目的是确定这种新的调节机制是否普遍适用于其他广泛不同种类细菌中硝酸盐呼吸的调节。在测试的12种细菌中，有11种全细胞悬液对硝酸盐的转运受到了完全且可逆的抑制，而无细胞提取物对硝酸盐的还原不受氧气影响，或在某些情况下仅受到部分抑制。这些结果表明，氧气对硝酸盐摄取的抑制是一种普遍的调节现象。
