Sundquist K T, Leppilampi M, Järvelin K, Kumpulainen T, Väänänen H K
Bone. 1987;8(1):33-8. doi: 10.1016/8756-3282(87)90129-3.
The presence of carbonic anhydrase isoenzymes I and II in rat monocytes and macrophagelike cells was studied using monospecific antisera against rat carbonic anhydrase I and II purified from red blood cells. CA II was strongly stained immunohistochemically in osteoclasts and macrophagelike cells in the umbilical cord. Foreign body giant cells, peritoneal macrophages, lung macrophages, and cultured peripheral monocytes, the presumed progenitor cells for osteoclasts, were negative with both antisera. Radioimmunoassay and immunoblotting similarly failed to demonstrate CA II in peripheral monocytes. The lack of CA in monocytes adds a new aspect to the discussion concerning the origin of osteoclasts and monocyte-mediated bone resorption.
利用针对从红细胞中纯化的大鼠碳酸酐酶I和II的单特异性抗血清，研究了大鼠单核细胞和巨噬细胞样细胞中碳酸酐酶同工酶I和II的存在情况。在破骨细胞和脐带中的巨噬细胞样细胞中，CA II经免疫组织化学强烈染色。异物巨细胞、腹腔巨噬细胞、肺巨噬细胞以及培养的外周单核细胞（推测为破骨细胞的祖细胞），两种抗血清检测均为阴性。放射免疫测定和免疫印迹同样未能在外周单核细胞中检测到CA II。单核细胞中缺乏CA为关于破骨细胞起源和单核细胞介导的骨吸收的讨论增添了新的内容。
