Zhejiang Provincial Center for Disease Control and Prevention, 3399 Bin Sheng Road, Binjiang District, Hangzhou, 310051, China.
Zhejiang Provincial Center for Disease Control and Prevention, 3399 Bin Sheng Road, Binjiang District, Hangzhou, 310051, China.
Chemosphere. 2019 Aug;229:160-168. doi: 10.1016/j.chemosphere.2019.05.016. Epub 2019 May 3.
To evaluate the body burdens of heavy metals correlated with health impact on school-age children living around a waste incinerator. A total of 81 children from the exposure area and 95 from the control area were recruited in our study. We measured the blood levels of chromium (Cr), cadmium (Cd) and lead (Pb) by an inductively coupled plasma mass spectrometer (ICP-MS), conducted comet assays, calculated the percentage of 5-methylcytosine (5 mC) and 5-hydroxymethylcytosine (5hmC) by MethylFlash methylated and a hydroxymethylated DNA quantification kit, and performed the flow cytometry to detect the expressions of surface antigens (including CD3, CD19, CD3CD4, CD3CD8, and CD3CD16 and/or CD56) in peripheral lymphocytes. Besides, we measured hormonal levels, including triiodothyronine (T3), thyroxine (T4), free triiodothyronine (FT3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH), and analyzed several regular hematological parameters. In addition, concentrations of heavy metals in environmental samples including rice, soils, vegetables, and drinking water were detected by ICP-MS. The mean blood levels of Cr, Cd, and Pb in the exposure group were all statistically higher than in the control group (2.57 vs. 0.79 μg/L; 1.83 vs. 1.81 μg/L; 44.00 vs. 32.31 μg/L, p < 0.01). The 5 mC and 5hmC levels in the exposure group were statistically lower (1.15% vs. 4.14%; 0.22% vs. 0.30%, p < 0.01), whereas the mean level of % tail DNA was statistically higher (10.10% vs. 8.62%, p < 0.01). Furthermore, the mean blood level of Cr and Pb was negatively correlated with the level of 5 mC (r = -0.279, r = -0.190, P < 0.05) in total population. In conclusion, children living in the vicinity of the municipal waste incinerator suffered increased body burdens of heavy metals (Cr, Cd, and Pb) associated with genotoxicity and epigenetic modifications.
为了评估与健康影响相关的重金属在居住在垃圾焚烧厂周围的学龄儿童体内的蓄积量。本研究共招募了暴露区的 81 名儿童和对照区的 95 名儿童。我们使用电感耦合等离子体质谱仪（ICP-MS）测量了血液中铬（Cr）、镉（Cd）和铅（Pb）的水平，进行彗星试验，使用 MethylFlash 甲基化和羟甲基化 DNA 定量试剂盒计算 5-甲基胞嘧啶（5mC）和 5-羟甲基胞嘧啶（5hmC）的百分比，并通过流式细胞术检测外周血淋巴细胞表面抗原（包括 CD3、CD19、CD3CD4、CD3CD8 和 CD3CD16 和/或 CD56）的表达。此外，我们还测量了激素水平，包括三碘甲状腺原氨酸（T3）、甲状腺素（T4）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）和促甲状腺激素（TSH），并分析了几项常规血液参数。此外，还使用 ICP-MS 检测了环境样本（包括大米、土壤、蔬菜和饮用水）中的重金属浓度。暴露组的 Cr、Cd 和 Pb 的平均血浓度均明显高于对照组（2.57 与 0.79μg/L；1.83 与 1.81μg/L；44.00 与 32.31μg/L，p<0.01）。暴露组的 5mC 和 5hmC 水平明显降低（1.15%与 4.14%；0.22%与 0.30%，p<0.01），而尾部 DNA 的平均水平明显升高（10.10%与 8.62%，p<0.01）。此外，在总人群中，Cr 和 Pb 的血浓度与 5mC 的水平呈负相关（r=-0.279，r=-0.190，P<0.05）。总之，居住在城市垃圾焚烧厂附近的儿童体内重金属（Cr、Cd 和 Pb）蓄积量增加，与遗传毒性和表观遗传修饰有关。
