Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, 211189, People's Republic of China.
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, 211189, People's Republic of China.
Biosens Bioelectron. 2019 Jul 15;137:45-51. doi: 10.1016/j.bios.2019.04.059. Epub 2019 May 1.
MicroRNAs (miRNAs) are regarded as a large variety of cancer-related biomarkers, and they have attracted wide attentions in recent years. In this work, a novel label-free strategy for the ultrasensitive detection of miRNA-182 (a typical biomarker for lung cancer) based on MoS/TiC nanohybrids was suggested. Firstly, modified glassy carbon electrode (GCE) with massive active sites and good electronic conductivity was prepared for biosensing. Then, based on this platform a descent signal in differential pulse voltammetry (DPV) peak current could be observed with the addition of probe RNA with negative charge. Thereafter, with the hybridization of target miRNA-182 with immobilized probe RNA and the swelling-induced breakage of Au-S bonds between RNA and the electrode surface, the characteristic DPV signals increase were found. In particular, this biosensing platform for special miRNAs possessed a good linear detection window in a range from 1 fM to 0.1 nM with a detection limit of 0.43 fM.
微小 RNA（miRNAs）被认为是一类广泛存在的癌症相关生物标志物，近年来受到了广泛关注。本工作提出了一种基于 MoS/TiC 纳米杂化材料的新型无标记策略，用于超灵敏检测 miRNA-182（一种典型的肺癌生物标志物）。首先，制备了具有大量活性位点和良好导电性的修饰玻碳电极（GCE），用于生物传感。然后，基于该平台，在差分脉冲伏安法（DPV）中观察到负电荷探针 RNA 加入后，电流信号显著下降。此后，随着目标 miRNA-182 与固定化探针 RNA 的杂交以及 RNA 与电极表面之间的 Au-S 键的肿胀诱导断裂，发现特征性的 DPV 信号增强。特别地，该用于特殊 miRNAs 的生物传感平台在 1 fM 至 0.1 nM 的范围内具有良好的线性检测窗口，检测限低至 0.43 fM。
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