Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, 211189, People's Republic of China.
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, 211189, People's Republic of China.
Biosens Bioelectron. 2019 Nov 1;144:111660. doi: 10.1016/j.bios.2019.111660. Epub 2019 Aug 29.
MicroRNA-155 (miRNA-155) is a typical cancer-related biomarker, which often exists at ultralow concentrations in the plasma or body fluids of early patients. In this work, a novel label-free platform for ultrasensitive miRNA-155 detection was designed based on the precise fabrication of molybdenum disulfide (MoS) by atomic layer deposition (ALD). Au nanoparticles (AuNPs)@MoS nanostructures were the core parts for the detection electrode, and the measurement precision of the sensing platform was modulated and optimized by ALD-based thickness and shape control of ultrathin MoS nanoflakes (thickness: ~14 nm, about 20 layers, uniform continuous distribution). In the detection experiment, MoS nanoflakes served as a conductive skeleton to support more AuNPs, and the results showed that the effective control of their morphology and thickness was of vital importance for ultrasensitive acquisition of detection signals. With using toluidine blue (TB) as a hybridization indicator, ultrasensitive detection record ranging from 1 fM to 10 nM with a detection limit of 0.32 fM can be achieved.
微小 RNA-155（miRNA-155）是一种典型的癌症相关生物标志物，通常在早期患者的血浆或体液中以超低浓度存在。在这项工作中，设计了一种基于原子层沉积（ALD）精确制造二硫化钼（MoS）的新型无标记平台，用于超灵敏 miRNA-155 检测。金纳米粒子（AuNPs）@MoS 纳米结构是检测电极的核心部分，通过基于 ALD 的超薄 MoS 纳米片的厚度和形状控制（厚度：~14nm，约 20 层，均匀连续分布）来调节和优化传感平台的测量精度。在检测实验中，MoS 纳米片作为导电骨架来支撑更多的 AuNPs，结果表明，对其形态和厚度的有效控制对于超灵敏检测信号的获取至关重要。使用甲苯胺蓝（TB）作为杂交指示剂，可以实现从 1fM 到 10nM 的超灵敏检测记录，检测限为 0.32fM。
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