Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Ocean College, Zhejiang University, Hangzhou 310058, China.
Mar Drugs. 2019 May 11;17(5):283. doi: 10.3390/md17050283.
The application of an OSMAC (One Strain-Many Compounds) approach on the sponge-derived fungus sp. LS34, using two different media including solid rice medium and potato dextrose broth (PDB) resulted in the isolation and identification of two new compounds, named asperspin A () and asperther A () along with seven known compounds -. Compounds - were detected in fungal extracts from rice medium, while compounds - were isolated from PDB medium. Their structures were unambiguously characterized by HRESIMS and NMR spectroscopic data. The growth inhibitory activity of these compounds against four pathogenic bacteria (, , , and ) were evaluated. All the compounds were also tested for their cytotoxicity against seven cancer cell lines, including CCRF-CEM, K562, BGC823, AGS, HCT-116, MDA-MB-453, and COR-L23. Among them, compound showed strong activity against CCRF-CEM and K562 cells with IC values of 1.22 ± 0.05 µM and 10.58 ± 0.19 µM, respectively. Notably, compound also showed pronounced activity against with an MIC value of 3.54 µM.
采用 OSMAC（一种菌株多种化合物）方法对海绵来源的真菌 sp. LS34 进行研究，使用两种不同的培养基，包括固体大米培养基和土豆葡萄糖肉汤（PDB），分离并鉴定出两种新化合物，命名为asperspin A () 和 asperther A () 以及七种已知化合物 - 。化合物 - 在大米培养基中的真菌提取物中检测到，而化合物 - 则从 PDB 培养基中分离得到。通过高分辨质谱（HRESIMS）和 NMR 波谱数据明确了它们的结构。评估了这些化合物对四种致病菌（ 、 、 、 和 ）的生长抑制活性。所有化合物还针对七种癌细胞系（包括 CCRF-CEM、K562、BGC823、AGS、HCT-116、MDA-MB-453 和 COR-L23）进行了细胞毒性测试。其中，化合物 对 CCRF-CEM 和 K562 细胞表现出较强的活性，IC 值分别为 1.22 ± 0.05 µM 和 10.58 ± 0.19 µM。值得注意的是，化合物 对 也表现出显著的活性，MIC 值为 3.54 µM。
Zotero 插件