Ahrari Farzaneh, Mazhari Fatemeh, Ghazvini Kiarash, Fekrazad Reza, Menbari Shaho, Nazifi Morteza
Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakilabad Blvd, Mashhad, Iran.
Dental Material Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Lasers Med Sci. 2023 Nov 10;38(1):260. doi: 10.1007/s10103-023-03914-y.
This study aimed to investigate the photodynamic effects of curcumin, nanomicelle curcumin, and erythrosine on Lactobacillus casei (L. casei). Various concentrations of curcumin (1.5 g/L, 3 g/L), nano-curcumin (3 g/L), and erythrosine (100 µM/L, 250 µM/L) were tested either alone or combined with light irradiation (PDT effect) against L. casei in planktonic and biofilm cultures. The light was emitted from a light-emitting diode (LED) with a central wavelength of 450 nm. A 0.12% chlorhexidine digluconate (CHX) solution served as the positive control, and a solution containing neither photosensitizer nor light was the negative control group. The number of viable microorganisms was determined using serial dilution. There was a significant difference in the viability of L. casei in both planktonic and biofilm forms (P < 0.05). In the planktonic culture, the antibacterial effects of CHX and PDT groups with curcumin 3 g/L and erythrosine 250 µM/L were significantly greater than the other groups (P < 0.05). For L. casei biofilms, the greatest toxic effects were observed in CHX and PDT groups with curcumin 3 g/L, erythrosine 250 µmol/L, erythrosine 100 µmol/L, and nanomicelle curcumin 3 g/L, with a significant difference to other groups (P < 0.05). The antibacterial effects of all photosensitizers (except erythrosine 250 µmol/L at planktonic culture) enhanced significantly when combined with light irradiation (P < 0.05). PDT with curcumin 3 g/L or erythrosine 250 µmol/L produced comparable results to CHX against L. casei at both planktonic and biofilm cultures. Alternatively, PDT with erythrosine 100 µmol/L or nanomicelle curcumin 3 g/L could be suggested to kill L. casei biofilms.
本研究旨在探究姜黄素、纳米胶束姜黄素和赤藓红对干酪乳杆菌的光动力效应。测试了不同浓度的姜黄素（1.5 g/L、3 g/L）、纳米姜黄素（3 g/L）和赤藓红（100 µM/L、250 µM/L）单独使用或与光照射联合使用（光动力疗法效应）对浮游和生物膜培养中的干酪乳杆菌的影响。光由中心波长为450 nm的发光二极管（LED）发出。0.12%的葡萄糖酸洗必泰（CHX）溶液作为阳性对照，不含光敏剂和光的溶液作为阴性对照组。采用系列稀释法测定活菌数。干酪乳杆菌在浮游和生物膜形式下的生存能力存在显著差异（P < 0.05）。在浮游培养中，CHX以及姜黄素3 g/L和赤藓红250 µM/L的光动力疗法组的抗菌效果显著高于其他组（P < 0.05）。对于干酪乳杆菌生物膜，在CHX以及姜黄素3 g/L、赤藓红250 µmol/L、赤藓红100 µmol/L和纳米胶束姜黄素3 g/L的光动力疗法组中观察到最大的毒性作用，与其他组有显著差异（P < 0.05）。当与光照射联合使用时，所有光敏剂（浮游培养中赤藓红250 µmol/L除外）的抗菌效果均显著增强（P < 0.05）。在浮游和生物膜培养中，姜黄素3 g/L或赤藓红250 µmol/L的光动力疗法对干酪乳杆菌产生的结果与CHX相当。另外，可建议使用赤藓红100 µmol/L或纳米胶束姜黄素3 g/L的光动力疗法来杀灭干酪乳杆菌生物膜。
