College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Plant Sci. 2019 Jul;284:127-134. doi: 10.1016/j.plantsci.2019.04.013. Epub 2019 Apr 17.
Avr9/Cf-9-INDUCED F-BOX1 (ACIF1) was first identified during screening of Avr9/Cf-9-elicited genes in tobacco. Further analysis revealed that ACIF1 was required for hypersensitive responses triggered by various elicitors in tobacco and tomato, indicating that it may be involved in various disease resistance. Here, we cloned its cotton (Gossypium hirsutum) homolog GhACIF1, which encodes an F-box protein. We show that GhACIF1 interacts with the putative SKP1-like protein, named GhSKP1. Disease resistance assays show that GhACIF1 enhances resistance to Verticillium dahliae in Arabidopsis plants, while silencing of GhACIF1 confers sensitivity to V. dahliae in cotton. Further analysis show that PevD1 elicitor activates hypersensitive and acquired immune response mediated by GhACIF1. Collectively, these results indicate that GhACIF1 contributes to protection against V. dahliae infection.
Avr9/Cf-9-INDUCED F-BOX1 (ACIF1) 是在筛选烟草中 Avr9/Cf-9 诱导基因时首次发现的。进一步的分析表明，ACIF1 是烟草和番茄中各种诱导剂引发的过敏反应所必需的，表明它可能参与了各种抗病性。在这里，我们克隆了棉花（Gossypium hirsutum）同源物 GhACIF1，它编码一个 F-box 蛋白。我们表明 GhACIF1 与假定的 SKP1 样蛋白 GhSKP1 相互作用。抗病性测定表明，GhACIF1 增强了拟南芥植物对黄萎病菌的抗性，而 GhACIF1 的沉默则使棉花对黄萎病菌敏感。进一步的分析表明，PevD1 诱导剂激活了由 GhACIF1 介导的过敏反应和获得性免疫反应。总的来说，这些结果表明 GhACIF1 有助于抵御黄萎病菌的感染。
