Geng Zhao, Liu Jianguang, Zhao Guiyuan, Geng Xiangli, Liu Xu, Liu Xingyu, Zhang Hanshuang, Wang Yongqiang
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050000, China.
Institute of Grain and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050000, China.
Int J Mol Sci. 2025 Jan 6;26(1):418. doi: 10.3390/ijms26010418.
SKP1 constitutes the Skp1-Cullin-F-box ubiquitin E3 ligase (SCF), which plays a role in plant growth and development and biotic and abiotic stress in ubiquitination. However, the response of the gene family to abiotic and biotic stresses in cotton has not been well characterized. In this study, a total of 72 genes with the conserved domain of SKP1 were identified in four Gossypium species. Synteny and collinearity analyses revealed that segmental duplication played a major role in the expansion of the cotton gene family. All SKP1-like proteins were classified into three different subfamilies via phylogenetic analysis. Furthermore, we focused on a comprehensive analysis of genes in . The cis-acting elements in the promoter site of the genes predict their involvement in multiple hormonal and defense stress responses. The expression patterns results indicated that 16 genes were expressed in response to biotic or abiotic stresses. To further validate the role of the genes in salt stress, four genes were randomly selected for gene silencing via VIGS. The results showed that the silencing of resulted in the inhibition of plant growth under salt stress, suggesting that was involved in the response to salt stress. In addition, yeast two-hybrid results revealed that proteins have different abilities to interact with F-box proteins. These results provide valuable information for elucidating the evolutionary relationships of the gene family and aiding further studies on the function of genes in cotton.
SKP1构成Skp1 - Culli - F - box泛素E3连接酶（SCF），其在植物生长发育以及泛素化介导的生物和非生物胁迫中发挥作用。然而，该基因家族对棉花生物和非生物胁迫的响应尚未得到充分表征。在本研究中，在四个棉属物种中总共鉴定出72个具有SKP1保守结构域的基因。共线性和同源性分析表明，片段重复在棉花基因家族的扩展中起主要作用。通过系统发育分析，所有SKP1样蛋白被分为三个不同的亚家族。此外，我们重点对某一物种中的基因进行了全面分析。这些基因启动子区域的顺式作用元件预示它们参与多种激素和防御胁迫反应。表达模式结果表明，16个基因响应生物或非生物胁迫而表达。为了进一步验证这些基因在盐胁迫中的作用，通过病毒诱导的基因沉默（VIGS）随机选择四个基因进行基因沉默。结果表明，基因沉默导致盐胁迫下植物生长受到抑制，表明该基因参与盐胁迫响应。此外，酵母双杂交结果显示，这些蛋白与F - box蛋白相互作用的能力不同。这些结果为阐明该基因家族的进化关系以及辅助进一步研究棉花中该基因的功能提供了有价值的信息。
