Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Heidelberg, Germany.
Department of Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg, Heidelberg University Hospital, Heidelberg, Germany.
Metabolomics. 2019 May 13;15(5):78. doi: 10.1007/s11306-019-1541-2.
Translational cancer research has seen an increasing interest in metabolomic profiling to decipher tumor phenotypes. However, the impact of post-surgical freezing delays on mass spectrometric metabolomic measurements of the cancer tissue remains elusive.
To evaluate the impact of post-surgical freezing delays on cancer tissue metabolomics and to investigate changes per metabolite and per metabolic pathway.
We performed untargeted metabolomics on three cortically located and bulk-resected glioblastoma tissues that were sequentially frozen as duplicates at up to six different time delays (0-180 min, 34 samples).
Statistical modelling revealed that 10% of the metabolome (59 of 597 metabolites) changed significantly after a 3 h delay. While carbohydrates and energy metabolites decreased, peptides and lipids increased. After a 2 h delay, these metabolites had changed by as much as 50-100%. We present the first list of metabolites in glioblastoma tissues that are sensitive to post-surgical freezing delays and offer the opportunity to define individualized fold change thresholds for future comparative metabolomic studies.
More researchers should take these pre-analytical factors into consideration when analyzing metabolomic data. We present a strategy for how to work with metabolites that are sensitive to freezing delays.
转化癌症研究越来越关注代谢组学分析，以破译肿瘤表型。然而，手术冷冻延迟对癌症组织的质谱代谢组学测量的影响仍不清楚。
评估手术冷冻延迟对癌症组织代谢组学的影响，并研究每个代谢物和每个代谢途径的变化。
我们对三个位于皮质并整块切除的胶质母细胞瘤组织进行了非靶向代谢组学分析，这些组织以最多 6 个不同的时间延迟（0-180 分钟，34 个样本）进行了连续冷冻。
统计建模显示，在 3 小时的延迟后，10%的代谢组（597 种代谢物中的 59 种）发生了显著变化。碳水化合物和能量代谢物减少，而肽和脂质增加。在 2 小时的延迟后，这些代谢物的变化高达 50-100%。我们首次提出了对手术冷冻延迟敏感的胶质母细胞瘤组织中的代谢物列表，并为未来的比较代谢组学研究提供了定义个性化倍数变化阈值的机会。
更多的研究人员在分析代谢组学数据时应考虑这些分析前因素。我们提出了一种处理对冷冻延迟敏感的代谢物的策略。
