Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
J Antimicrob Chemother. 2019 Aug 1;74(8):2171-2175. doi: 10.1093/jac/dkz199.
Fully sequenced IncI1 plasmids obtained from CTX-M-1-producing Escherichia coli of human and animal origin were compared.
Twelve E. coli isolates sharing identical ESBL genes and plasmid multilocus STs sequenced on Illumina and MinION platforms were obtained from the Danish antimicrobial resistance surveillance programme, DANMAP. After de novo assembly, the sequences of plasmids harbouring blaCTX-M-1 were manually curated and ORFs annotated. Within-group comparisons were performed separately for the IncI1 ST3 plasmid type and the IncI1 ST7 plasmid type. The IncI1 ST3 plasmid group was obtained from 10 E. coli isolates (2 from patients with bloodstream infections, 6 from food and 2 from animals). The IncI1 ST7 plasmids originated from E. coli isolates obtained from a patient with bloodstream infection and from a pig. Sequences of IncI1 ST3 and IncI1 ST7 plasmids harbouring blaCTX-M-1 with determined origin were retrieved from GenBank and used for comparison within the respective group.
The 10 IncI1 ST3 blaCTX-M-1 plasmids were highly similar in structure and organization with only minor plasmid rearrangements and differences in the variable region. The IncI1 ST7 blaCTX-M-1 plasmids also showed high similarity in structure and organization. The high level of similarity was also observed when including plasmids from E. coli of animal origin from Australia, Switzerland, the Netherlands and France.
This study shows broad spread of a very successful CTX-M-1-producing IncI1 type plasmid among E. coli of both human and animal origin.
比较来自人源和动物源产 CTX-M-1 的大肠杆菌的完全测序 IncI1 质粒。
从丹麦抗菌药物耐药监测计划（DANMAP）中获得了 12 株携带相同 ESBL 基因和质粒多位点 ST 的大肠杆菌分离株，这些分离株在 Illumina 和 MinION 平台上进行了测序。在从头组装后，人工整理了携带 blaCTX-M-1 的质粒序列，并对 ORFs 进行了注释。分别对 IncI1 ST3 质粒和 IncI1 ST7 质粒进行了组内比较。IncI1 ST3 质粒组来自 10 株大肠杆菌分离株（2 株来自血流感染患者，6 株来自食品，2 株来自动物）。IncI1 ST7 质粒来自血流感染患者和猪的大肠杆菌分离株。从 GenBank 中检索到携带 blaCTX-M-1 的 IncI1 ST3 和 IncI1 ST7 质粒的序列，确定其来源，并在各自的组内进行比较。
10 株 IncI1 ST3 blaCTX-M-1 质粒在结构和组织上高度相似，只有微小的质粒重排和可变区的差异。IncI1 ST7 blaCTX-M-1 质粒在结构和组织上也表现出高度相似。当包括来自澳大利亚、瑞士、荷兰和法国的动物源大肠杆菌的质粒时，也观察到了高度的相似性。
本研究表明，一种非常成功的产 CTX-M-1 的 IncI1 型质粒在人源和动物源大肠杆菌中广泛传播。
