氧化型高密度脂蛋白/高密度脂蛋白胆固醇比值升高作为超重和肥胖个体代谢健康紊乱的潜在指标。

Increased Oxidized High-Density Lipoprotein/High-Density Lipoprotein-Cholesterol Ratio as a Potential Indicator of Disturbed Metabolic Health in Overweight and Obese Individuals.

作者信息

Janac Jelena M, Zeljkovic Aleksandra, Jelic-Ivanovic Zorana D, Dimitrijevic-Sreckovic Vesna S, Vekic Jelena, Miljkovic Milica M, Stefanovic Aleksandra, Kotur-Stevuljevic Jelena M, Ivanisevic Jasmina M, Spasojevic-Kalimanovska Vesna V

机构信息

Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Republic of Serbia.

Clinic for Endocrinology, Diabetes and Metabolic Diseases, Clinical Center of Serbia; Faculty of Medicine, University of Belgrade, Republic of Serbia.

出版信息

Lab Med. 2020 Jan 2;51(1):24-33. doi: 10.1093/labmed/lmz017.

DOI:10.1093/labmed/lmz017

PMID:31089722

Abstract

BACKGROUND

We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects.

METHODS

The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis.

RESULTS

An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes.

CONCLUSIONS

Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.

摘要

背景

我们评估了代谢健康和不健康的超重及肥胖受试者中高密度脂蛋白（HDL）颗粒的定性特征。

方法

该研究纳入了115名分为代谢健康和不健康组的超重及肥胖受试者个体。使用商业酶联免疫吸附测定（ELISA）试剂盒测量氧化型HDL（OxHDL）和血清淀粉样蛋白A（SAA）浓度。采用非变性梯度凝胶电泳分离脂蛋白亚组分。

结果

超重和肥胖组中，OxHDL/HDL-胆固醇比值升高与代谢不健康表型的发生之间存在独立关联。OxHDL/HDL-胆固醇比值在确定代谢健康表型方面显示出优异且可接受的诊断准确性（超重组，AUC = 0.881；肥胖组，AUC = 0.765）。通过脂蛋白亚组分分析证实了代谢不健康受试者中较小HDL颗粒的积累。不同表型之间SAA浓度无显著差异。