Division of Endocrinology and Molecular Medicine, University of Kentucky, Lexington, Kentucky, USA.
Obesity (Silver Spring). 2013 May;21(5):993-6. doi: 10.1002/oby.20126.
In murine models of obesity/diabetes, there is an increase in plasma serum amyloid A (SAA) levels along with redistribution of SAA from high-density lipoprotein (HDL) to apolipoprotein B (apoB)-containing lipoprotein particles, namely, low-density lipoprotein and very low-density lipoprotein. The goal of this study was to determine if obesity is associated with similar SAA lipoprotein redistribution in humans.
Three groups of obese individuals were recruited from a weight loss clinic: healthy obese (n = 14), metabolic syndrome (MetS) obese (n = 8), and obese with type 2 diabetes (n = 6). Plasma was separated into lipoprotein fractions by fast protein liquid chromatography, and SAA was measured in lipid fractions using enzyme-linked immunosorbent assay and Western blotting.
Only the obese diabetic group had SAA detectable in apoB-containing lipoproteins, and SAA reverted back to HDL with active weight loss.
In human subjects, SAA is found in apoB-containing lipoprotein particles only in obese subjects with type 2 diabetes, but not in healthy obese or obese subjects with MetS.
在肥胖/糖尿病的小鼠模型中，血浆血清淀粉样蛋白 A（SAA）水平升高，同时 SAA 从高密度脂蛋白（HDL）重新分布到载有载脂蛋白 B（apoB）的脂蛋白颗粒，即低密度脂蛋白和极低密度脂蛋白。本研究旨在确定肥胖是否与人类类似的 SAA 脂蛋白重新分布有关。
从减肥诊所招募了三组肥胖个体：健康肥胖者（n = 14）、代谢综合征肥胖者（n = 8）和 2 型糖尿病肥胖者（n = 6）。使用快速蛋白液相色谱法将血浆分离成脂蛋白级分，并使用酶联免疫吸附试验和 Western 印迹法测量脂质级分中的 SAA。
只有肥胖糖尿病组的 apoB 载脂蛋白中可检测到 SAA，而积极减肥后 SAA 又回到了 HDL。
在人体中，只有 2 型糖尿病肥胖者的 apoB 载脂蛋白中才发现 SAA，而健康肥胖者或代谢综合征肥胖者中则没有。
