Lanir A, Clouse M E, Lee R G
Transplantation. 1987 Jun;43(6):786-90.
31P NMR spectroscopy proved to be an excellent, dynamic, nondestructive method for assessing the liver during cold flush and pulsatile perfusion experiments. 31P NMR spectroscopy was used to measure ATP decay, inorganic phosphate appearance, and phosphate chemical shift in the excised mouse livers subjected to cold and warm ischemia. Cold flush followed by cold preservation in saline, Krebs-Henseleit buffer, or Collins' solution showed that Collins' solution resulted in the slowest ATP decay. In temperature-controlled experiments (5 degrees -37 degrees C), ATP decay was much slower with lower temperature. In separate pulsatile perfusion experiments with oxygenated Krebs-Henseleit buffer, hepatic ATP was unchanged for at least 6 hr at 20 degrees C. At 37 degrees C, the NMR spectrum showed changes in the diphosphoesters region, but the ATP remained stable during the 6-hr perfusion. These studies suggest that for long periods of liver preservation, an adequate perfusion method should be developed.
31P核磁共振波谱法被证明是一种在冷冲洗和搏动灌注实验中评估肝脏的出色、动态、无损的方法。31P核磁共振波谱法用于测量经受冷缺血和热缺血的切除小鼠肝脏中的ATP衰减、无机磷酸盐出现以及磷酸盐化学位移。冷冲洗后在生理盐水、克雷布斯-亨塞尔特缓冲液或柯林斯溶液中进行冷保存表明，柯林斯溶液导致ATP衰减最慢。在温度控制实验（5摄氏度-37摄氏度）中，温度越低，ATP衰减越慢。在使用含氧克雷布斯-亨塞尔特缓冲液的单独搏动灌注实验中，肝脏ATP在20摄氏度下至少6小时保持不变。在37摄氏度时，核磁共振谱显示二磷酸酯区域有变化，但在6小时灌注期间ATP保持稳定。这些研究表明，为了进行长时间的肝脏保存，应开发一种适当的灌注方法。
