Laboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Brazil.
Helicobacter. 2020 Feb;25(1):e12667. doi: 10.1111/hel.12667. Epub 2019 Nov 8.
IL-27 has dual roles in the immune response either stimulating Th1 or inhibiting Th17 cells. Because there is a particular link of IL-23/Th17 axis in the development of cancer and IL-27 has been considered a potential treatment for cancer, we evaluated the gastric and serum concentrations of IL-27 in two mutually exclusive Helicobacter pylori-associated diseases, gastric cancer (GC) and duodenal ulcer (DU).
We prospectively studied 110 H pylori-positive patients and 40 healthy blood donors. Serum and gastric concentrations of IL-27 and cytokines of the Th1/Th17 cells were assessed by ELISA.
IL-27 was not detected in GC patients, but the cytokine concentration was very high in the patients with DU. IL-27 was also detected in the gastritis patients and in the H pylori-positive blood donors. IL27RA mRNA expression in peripheral blood mononuclear cells, evaluated by rt-PCR, was stimulated by H pylori strains. The cytokine concentration positively correlated with the Th1 and negatively with Th17 cell representative cytokine levels. Gastric IL-27 concentrations were positively correlated with increased degree of mononuclear and polymorphonuclear cells on the antral gastric mucosa of DU patients in consonance with the DU gastritis pattern. IL-12p70 and IFN-γ gastric concentrations were significantly higher in DU than in GC. Conversely, gastric concentrations of Th17 cell-associated cytokines (IL-1β, IL-6, IL-17A, IL-23, and TGF-β) were significantly higher in GC than in DU patients.
Although H pylori infection is able to elicit IL-27 and IL-27Rα secretion, DU and GC have diametrically opposed cytokine patterns.
IL-27 在免疫反应中具有双重作用，既能刺激 Th1 细胞，也能抑制 Th17 细胞。由于 IL-23/Th17 轴在癌症的发展中存在特定联系，并且 IL-27 已被认为是癌症的一种潜在治疗方法，因此我们评估了两种相互排斥的幽门螺杆菌相关疾病（胃癌和十二指肠溃疡）中 IL-27 在胃和血清中的浓度。
我们前瞻性研究了 110 例 H pylori 阳性患者和 40 名健康献血者。通过 ELISA 评估血清和胃组织中 IL-27 和 Th1/Th17 细胞细胞因子的浓度。
GC 患者中未检测到 IL-27，但 DU 患者的细胞因子浓度非常高。胃炎患者和 H pylori 阳性献血者中也检测到了 IL-27。通过 rt-PCR 评估外周血单个核细胞中的 IL27RA mRNA 表达，发现其受到 H pylori 菌株的刺激。细胞因子浓度与 Th1 呈正相关，与 Th17 细胞代表性细胞因子水平呈负相关。DU 患者胃组织中 IL-27 浓度与胃窦黏膜中单核细胞和多形核细胞的增加程度呈正相关，与 DU 胃炎模式一致。DU 患者的胃组织中 IL-12p70 和 IFN-γ 浓度明显高于 GC 患者。相反，GC 患者的胃组织中 Th17 细胞相关细胞因子（IL-1β、IL-6、IL-17A、IL-23 和 TGF-β）浓度明显高于 DU 患者。
尽管 H pylori 感染能够引发 IL-27 和 IL-27Rα 的分泌，但 DU 和 GC 具有截然相反的细胞因子模式。
