Singh Ranjana, Kumar Pramod, Mishra Devendra Nath, Singh Ashok Kumar, Singh Raj Kumar, Mahdi Abbas Ali, Gierke Cathy Lee, Cornelissen Germaine
1Biochemistry Department, King George's Medical University, Lucknow, 226003 India.
Medicine Department, T S Misra Medical College and Hospital, Amausi, Lucknow, 226008 India.
Indian J Clin Biochem. 2019 Apr;34(2):164-171. doi: 10.1007/s12291-017-0724-8. Epub 2017 Dec 21.
The circadian rhythm of uric acid concentration was studied under near-normal tropical conditions in 162 healthy volunteers (103 males and 59 females; 7 to 75 year). They were mostly medical students, staff members and members of their families. They were classified into 4 age groups: A (7-20 y; N = 42), B (21-40 y; N = 60), C (41-60 y; N = 35) and D (61-75 y; N = 25). They followed a diurnal activity from about 06:00 to about 22:00 and nocturnal rest. Blood samples were collected from each subject every 6 for 24 h (4 samples). Serum uric acid was measured spectrophotometrically. Data from each subject were analyzed by cosinor rhythmometry. Effects of gender, age, diet (vegetarian vs. omnivore), and smoking status on the rhythm-adjusted mean (MESOR) and circadian amplitude were examined by multiple-analysis of variance. A marked circadian variation was found in uric acid concentration in healthy Indians of all age groups. Furthermore, both the MESOR and circadian amplitude underwent changes with advancing age. In addition to effects of gender and age, diet and smoking were also found to affect the MESOR of circulating uric acid concentration in healthy Indians residing in northern India. The present observations confirmed a definite rhythm in uric acid concentrations with significant effect of gender, age, diet, and smoking status on uric acid concentration in clinical health. Mapping the circadian rhythm of serum uric acid is needed to explore their role in different pathophysiological conditions.
在接近正常的热带条件下，对162名健康志愿者（103名男性和59名女性；年龄在7至75岁之间）的尿酸浓度昼夜节律进行了研究。他们大多是医学生、工作人员及其家属。他们被分为4个年龄组：A组（7 - 20岁；N = 42）、B组（21 - 40岁；N = 60）、C组（41 - 60岁；N = 35）和D组（61 - 75岁；N = 25）。他们遵循从大约06:00至大约22:00的日间活动和夜间休息模式。每6小时从每个受试者采集一次血样，共采集24小时（4个样本）。采用分光光度法测定血清尿酸。对每个受试者的数据进行余弦节律分析法分析。通过多因素方差分析研究性别、年龄、饮食（素食者与杂食者）和吸烟状况对节律调整均值（MESOR）和昼夜振幅的影响。在所有年龄组的健康印度人中，尿酸浓度均呈现出明显的昼夜变化。此外，MESOR和昼夜振幅均随年龄增长而发生变化。除了性别和年龄的影响外，还发现饮食和吸烟也会影响居住在印度北部的健康印度人循环尿酸浓度的MESOR。本观察结果证实了尿酸浓度存在明确的节律，性别、年龄、饮食和吸烟状况对临床健康状态下的尿酸浓度有显著影响。需要绘制血清尿酸的昼夜节律图，以探索其在不同病理生理状况中的作用。
