J Adhes Dent. 2019;21(3):229-237. doi: 10.3290/j.jad.a42521.
To investigate the use of a cold atmospheric plasma (CAP) source in a clinically realistic endodontic procedure to enhance the bond strength of a dental adhesive in root canal restoration.
CAP was generated by a handheld DBD-jet prototype specifically designed for biomedical applications. Extracted monoradicular teeth were standardized through crown sectioning and root canal shaping before being embedded in epoxy resin cylinders using a custom molding procedure designed to ensure the accurate alignment of the specimens. Afterwards, the dentin surface was conditioned according to different protocols including (or not, in controls) chelating agents (EDTA or IP6) and CAP treatment (180 s). Then a self-etch adhesive was applied, followed by a luting material to seal the root canal. Both materials were light cured. Tooth sections were obtained from coronal and middle portions of the root canal, and the push-out test was used to evaluate the bond strength between the adhesive and dentin.
Push-out results demonstrated that plasma treatment greatly improved (> twofold) the mechanical properties of the adhesive-dentin interface along the whole length of the root canal. Contact angle measurements and SEM analyses showed that plasma treatment facilitated adhesive permeation into dentinal tubules, hence enhancing the effects of the bonding procedure.
Although investigations on long-term bond stability after CAP treatment and clinical studies are required, the present study indicates that CAP devices may be useful in clinical endodontic treatment.
研究在临床实际的根管治疗过程中使用冷等离体（CAP）源来增强牙本质根管修复中牙科胶粘剂的粘结强度。
使用专门为生物医学应用设计的手持式 DBD-jet 原型机产生 CAP。通过对牙冠进行分段和根管成型，对提取的单根管牙齿进行标准化处理，然后使用定制的成型程序将其嵌入环氧树脂圆柱体中，该程序设计用于确保标本的精确对准。之后，根据不同的方案对牙本质表面进行处理，包括（或不包括对照组中的）螯合剂（EDTA 或 IP6）和 CAP 处理（180 s）。然后应用自酸蚀胶粘剂，再用粘固材料密封根管。两种材料都经过光固化处理。从根管的冠部和中部切取牙段，并进行推出试验以评估胶粘剂与牙本质之间的粘结强度。
推出试验结果表明，等离子体处理极大地提高了（两倍以上）整个根管长度上胶粘剂与牙本质界面的机械性能。接触角测量和 SEM 分析表明，等离子体处理促进了胶粘剂渗透到牙本质小管中，从而增强了粘结过程的效果。
尽管需要对 CAP 处理后的长期粘结稳定性进行调查和临床研究，但本研究表明 CAP 设备可能在临床牙髓治疗中有用。
