Department of Research and Development, FEAGLE Corporations, 70-6, Jeungsan-ro, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do, 50614, South Korea.
Department of Oral Anatomy and Cell Biology, School of Dentistry, Pusan National University, Busan, South Korea.
Sci Rep. 2022 May 9;12(1):7597. doi: 10.1038/s41598-022-11665-z.
To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 10 colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future.
为了将低温等离子体的灭菌效果应用于口腔，必须解决等离子体中的臭氧问题。在这项研究中，开发了一种产生几乎不含臭氧的冷等离子体的新技术，并将其命名为 Nozone（无臭氧）冷等离子体（NCP）技术。测试了 NCP 对四种口腔病原体的抗菌效果，并阐明了其具体机制。NCP 对固体培养基上的口腔致病微生物的处理产生了生长抑制区。当 NCP 应用于液体培养基中的口腔病原体时，微生物的生长减少了超过 10 个菌落形成单位，并且在安装牙尖后 NCP 仍然具有杀菌作用。NCP 在液体培养基中的杀菌作用是由于培养基中过氧化氢水平的增加。然而，NCP 在固体培养基中的杀菌作用取决于 NCP 的带电元素。此外，安装牙尖的 NCP 的表面杀菌效率与牙尖的孔径成正比，与牙尖的长度成反比。总的来说，我们预计这种 NCP 装置在不久的将来将在牙科中得到广泛应用。
