Department of Neurology, Shengjing Hospital of China Medical University, Shenyang, Liao Ning, China.
Multidisciplinary Neuroprotection Laboratories, Departments of Anesthesiology, Biomedical Engineering, Neurobiology, and Neurosurgery, Duke University, Durham, NC.
Crit Care Med. 2019 Aug;47(8):e693-e699. doi: 10.1097/CCM.0000000000003809.
We tested the hypothesis that prolonged inhalation of 70% argon for 24 hours after in vivo permanent or temporary stroke provides neuroprotection and improves neurologic outcome and overall recovery after 7 days.
Controlled, randomized, double-blinded laboratory study.
Animal research laboratories.
Adult Wistar male rats (n = 110).
Rats were subjected to permanent or temporary focal cerebral ischemia via middle cerebral artery occlusion, followed by inhalation of 70% argon or nitrogen in 30% oxygen for 24 hours. On postoperative day 7, a 48-point neuroscore and histologic lesion size were assessed.
After argon inhalation for 24 hours immediately following "severe permanent ischemia" induction, neurologic outcome (neuroscore, p = 0.034), overall recovery (body weight, p = 0.02), and infarct volume (total infarct volume, p = 0.0001; cortical infarct volume, p = 0.0003; subcortical infarct volume, p = 0.0001) were significantly improved. When 24-hour argon treatment was delayed for 2 hours after permanent stroke induction or until after postischemic reperfusion treatment, neurologic outcomes remained significantly improved (neuroscore, p = 0.043 and p = 0.014, respectively), as was overall recovery (body weight, p = 0.015), compared with nitrogen treatment. However, infarct volume and 7-day mortality were not significantly reduced when argon treatment was delayed.
Neurologic outcome (neuroscore), overall recovery (body weight), and infarct volumes were significantly improved after 24-hour inhalation of 70% argon administered immediately after severe permanent stroke induction. Neurologic outcome and overall recovery were also significantly improved even when argon treatment was delayed for 2 hours or until after reperfusion.
我们检验了这样一个假设，即在体内永久性或暂时性中风后持续吸入 70%的氩气 24 小时可提供神经保护，并改善 7 天后的神经功能预后和整体恢复情况。
对照、随机、双盲实验室研究。
动物研究实验室。
成年 Wistar 雄性大鼠（n=110）。
通过大脑中动脉闭塞使大鼠发生永久性或暂时性局灶性脑缺血，随后在 30%氧气中吸入 70%的氩气或氮气 24 小时。在术后第 7 天，评估 48 分神经评分和组织学损伤大小。
在“严重永久性缺血”诱导后立即吸入 24 小时氩气后，神经功能预后（神经评分，p=0.034）、整体恢复（体重，p=0.02）和梗死体积（总梗死体积，p=0.0001；皮质梗死体积，p=0.0003；皮质下梗死体积，p=0.0001）均显著改善。当 24 小时氩气治疗延迟至永久性中风诱导后 2 小时或直到缺血后再灌注治疗时，与氮气治疗相比，神经功能预后仍显著改善（神经评分，p=0.043 和 p=0.014），整体恢复也得到改善（体重，p=0.015）。然而，当氩气治疗延迟时，梗死体积和 7 天死亡率并未显著降低。
在严重永久性中风诱导后立即吸入 70%的氩气 24 小时，可显著改善神经功能预后（神经评分）、整体恢复（体重）和梗死体积。即使氩气治疗延迟 2 小时或直到再灌注后，神经功能预后和整体恢复仍有显著改善。
