Bonini S, Trocmé S D, Barney N P, Brash P C, Bloch K J, Allansmith M R
Curr Eye Res. 1987 May;6(5):659-65. doi: 10.3109/02713688709034828.
Tear fluid cytology is described for the early and late phases of ocular anaphylaxis in actively immunized Sprague-Dawley rats. Tears were collected from both eyes of the rats before challenge and at 1, 2, 4, 6, 8, 10, 12, 24, and 48 h after topical challenge with di-DNP-lysine in one eye and PBS in the fellow eye. Results showed a statistically significant increase in the Aggregate Cell Rating, which represents the aggregate scores in neutrophil, eosinophil, lymphocyte, and atypical epithelial cell levels, in antigen-treated vs control eyes. This report is the first to use a cytologic study of tear film to detect the late phase of ocular anaphylaxis in the rat. Cytology of the tear film could be applied to the study of allergic conjunctivitis in both animals and humans.
本文描述了在主动免疫的斯普拉格-道利大鼠眼部过敏反应的早期和晚期阶段的泪液细胞学情况。在对大鼠一只眼局部注射二硝基苯赖氨酸（di-DNP-lysine），另一只眼注射磷酸盐缓冲液（PBS）进行激发之前以及激发后1、2、4、6、8、10、12、24和48小时，从大鼠双眼收集泪液。结果显示，与对照眼相比，抗原处理眼的总细胞评分有统计学显著增加，总细胞评分代表中性粒细胞、嗜酸性粒细胞、淋巴细胞和非典型上皮细胞水平的总分。本报告首次利用泪膜细胞学研究来检测大鼠眼部过敏反应的晚期阶段。泪膜细胞学可应用于动物和人类过敏性结膜炎的研究。
