Trocmé S D, Bonini S, Barney N P, Bloch K J, Allansmith M R
Eye Research Institute of Retina Foundation, Massachusetts General Hospital, Boston.
Acta Ophthalmol (Copenh). 1988 Feb;66(1):24-32. doi: 10.1111/j.1755-3768.1988.tb08529.x.
We tested the effects of pre-treatment with dexamethasone on topically induced ocular anaphylaxis in the rat. Rats were immunized with dinitrophenylated Ascaris suum extract and challenged with di-DNP-lysine. Dexamethasone was administered topically once (24, 6, or 1 h before challenge) or three times (6, 4, and 2 h before challenge). A single pre-treatment given at 24 or 6 h had no significant effect. A single pre-treatment 1 h before challenge reduced the extent of edema assessed histologically but not clinically, and had no significant effect on the eosinophil count in conjunctival tissue examined 6 h after challenge. Eyes pre-treated with dexamethasone 6, 4, and 2 h before challenge showed a significant reduction in conjunctival edema assessed histologically and clinically 1 h after challenge. In addition, 6 h after challenge the number of eosinophils was significantly reduced. We conclude that repeated pre-treatment with dexamethasone can suppress both the immediate phase and the cellular late phase of topically induced ocular anaphylaxis.
我们测试了地塞米松预处理对大鼠局部诱导的眼部过敏反应的影响。用二硝基苯基化猪蛔虫提取物对大鼠进行免疫，并用二 - DNP - 赖氨酸进行激发。地塞米松在激发前一次（激发前24、6或1小时）或三次（激发前6、4和2小时）局部给药。在24或6小时进行单次预处理没有显著效果。激发前1小时进行单次预处理可使组织学评估的水肿程度减轻，但临床评估无此效果，且对激发后6小时检查的结膜组织中的嗜酸性粒细胞计数没有显著影响。在激发前6、4和2小时用地塞米松预处理的眼睛在激发后1小时组织学和临床评估的结膜水肿均显著减轻。此外，激发后6小时嗜酸性粒细胞数量显著减少。我们得出结论，地塞米松重复预处理可抑制局部诱导的眼部过敏反应的速发相和细胞迟发相。
