Funderburk S J, Klisak I, Law M L, Ma N, Neiswanger K, Sparkes R S
Chromosoma. 1987;95(2):148-53. doi: 10.1007/BF00332188.
A dispersed middle repetitive DNA sequence isolated originally from human chromosome 12 did not show homology with rodent DNA under standard conditions of Southern DNA blot analysis. The evolutionary relationship of this human repetitive DNA to that of other primates was investigated using three hybridization methods: DNA dot blot, Southern DNA blot analysis, and chromosome in situ hybridization. Homology with the human repetitive DNA was found throughout the suborder Anthropoidea, in fourteen ape and New and Old World monkey species. In addition, the human pattern of hybridization to noncentromeric regions of all chromosomes was seen. No hybridization by any of the three techniques was found in five species of the suborder Prosimii. The phenomenon of marked differences in sequence homology and copy number of dispersed repetitive DNA from closely related species has been observed in protozoans (Plasmodia), Drosophila, sea urchins, mice and the great apes (Hominoidea). We report here a similar phenomenon that may have occurred at an early stage in primate evolution.
最初从人类12号染色体分离出的一个分散的中度重复DNA序列，在标准的Southern DNA印迹分析条件下，未显示与啮齿动物DNA具有同源性。使用三种杂交方法研究了这种人类重复DNA与其他灵长类动物重复DNA的进化关系：DNA斑点印迹、Southern DNA印迹分析和染色体原位杂交。在整个类人猿亚目、14种猿以及新大陆和旧大陆猴物种中，均发现与人类重复DNA具有同源性。此外，还观察到人类与所有染色体非着丝粒区域的杂交模式。在原猴亚目5个物种中，未发现这三种技术中的任何一种有杂交现象。在原生动物（疟原虫）、果蝇、海胆、小鼠和类人猿（人猿总科）中，已观察到密切相关物种的分散重复DNA在序列同源性和拷贝数上存在显著差异的现象。我们在此报告一种可能在灵长类动物进化早期就已出现的类似现象。
