人参皂苷 Rb1 通过 PI3K/AKT/GSK-3β 信号通路促进水貂毛囊的生长。
Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway.
机构信息
College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, People's Republic of China; State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, People's Republic of China.
College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, People's Republic of China.
出版信息
Life Sci. 2019 Jul 15;229:210-218. doi: 10.1016/j.lfs.2019.05.033. Epub 2019 May 16.
AIMS
Hair follicles play a critical role in the process of hair growth. The dermal papilla cells (DPCs) are an important component in the hair follicle regeneration and growth. This study investigated the effects of ginsenoside Rb1 on the growth of cultured mink hair follicles and DPCs.
MAIN METHODS
The mink hair follicles were treated with ginsenoside Rb1 for 9 days and their lengths were measured every three days. Real-time PCR was used to determine the mRNA expression of vascularization endothelial growth factor A (VEGF-A), VEGF receptor 2 (VEGF-R2) and TGF-β1. In addition, the levels of proteins were detected by western blot. Cell proliferation was determined by immunofluorescence staining of proliferation marker Ki-67 and cell cycle analysis was performed on flow cytometry. Moreover, cell migration was evaluated by wound healing assay.
KEY FINDINGS
Ginsenoside Rb1 promoted the growth of hair follicles, and proliferation and migration of DPCs. Ginsenoside Rb1 improved the expression levels of VEGFA and VEGF-R2, while attenuated the TGF-β1 expression both in hair follicles and DPCs. Furthermore, ginsenoside Rb1 facilitated the activation of PI3K/AKT/GSK-3β signaling pathway in hair follicles and DPCs.
SIGNIFICANCE
The results reveals a crucial role of PI3K/AKT/GSK-3β signaling pathway in ginsenoside Rb1-induced growth of hair follicles and DPCs.
目的
毛囊在毛发生长过程中起着关键作用。真皮乳头细胞（DPCs）是毛囊再生和生长的重要组成部分。本研究探讨了人参皂苷 Rb1 对培养的水貂毛囊和 DPCs 生长的影响。
主要方法
用人参皂苷 Rb1 处理水貂毛囊 9 天，每 3 天测量一次长度。实时 PCR 用于测定血管生成内皮生长因子 A（VEGF-A）、VEGF 受体 2（VEGF-R2）和 TGF-β1 的 mRNA 表达。此外，通过 Western blot 检测蛋白水平。增殖标志物 Ki-67 的免疫荧光染色用于检测细胞增殖，通过流式细胞术进行细胞周期分析。此外，通过划痕愈合试验评估细胞迁移。
主要发现
人参皂苷 Rb1 促进了毛囊、DPCs 的生长、增殖和迁移。人参皂苷 Rb1 提高了毛囊和 DPCs 中 VEGFA 和 VEGF-R2 的表达水平，同时减弱了 TGF-β1 的表达。此外，人参皂苷 Rb1 促进了毛囊和 DPCs 中 PI3K/AKT/GSK-3β 信号通路的激活。
意义
研究结果揭示了 PI3K/AKT/GSK-3β 信号通路在人参皂苷 Rb1 诱导的毛囊和 DPCs 生长中的重要作用。
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