Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China.
Institute of Health Food of Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Food Res Int. 2019 Jul;121:20-27. doi: 10.1016/j.foodres.2019.03.001. Epub 2019 Mar 4.
In order to investigate the synergistic effect between umami peptides and monosodium glutamate (MSG), a new assessment method was established using electronic tongue. After 36 kinds of umami peptides synthesized by peptide solid phase synthesis, their taste characteristics were preliminarily explored by electronic tongue technology, and then the umami intensity was ranked before and after addition of MSG, using a concentration of 0.35% of MSG as control. In addition, the sensory evaluation was utilized to verify the results of the electronic tongue. Finally, the umami intensity and the synergistic effect of umami peptides and MSG were also investigated by the aroma chicken model (ACM). Results showed that peptide Lys-Gly-Ser-Leu-Ala-Asp-Glu-Glu (KE-8) and Arg-Leu (RL) have the strongest umami taste, Asp-Asp-Asp (DDD) and Glu-Ser-Val (ESV) have the strongest synergistic effect with MSG, which could increase the umami intensity. The ESV and Glu-Asp-Asp (EDD) showed the strongest synergistic effect with ACM. The evaluation method could provide the objective data for further investigating for the synergistic theory.
为了研究鲜味肽与谷氨酸钠(MSG)之间的协同作用,采用电子舌建立了一种新的评价方法。通过多肽固相合成合成了 36 种鲜味肽,利用电子舌技术初步探讨了它们的味觉特征,然后在添加 0.35%MSG 的情况下,对MSG 前后的鲜味强度进行了排序,以 0.35%MSG 作为对照。此外,利用感官评价验证了电子舌的结果。最后,通过鸡肉香味模型(ACM)研究了鲜味肽和 MSG 的鲜味强度和协同作用。结果表明,肽 Lys-Gly-Ser-Leu-Ala-Asp-Glu-Glu(KE-8)和 Arg-Leu(RL)具有最强的鲜味,Asp-Asp-Asp(DDD)和 Glu-Ser-Val(ESV)与 MSG 具有最强的协同作用,可以提高鲜味强度。ESV 和 Glu-Asp-Asp(EDD)与 ACM 表现出最强的协同作用。该评价方法可为进一步研究协同理论提供客观数据。