Syakti Agung Dhamar, Lestari Priyati, Simanora Satya, Sari Lilik Kartika, Lestari Febrianti, Idris Fadliyah, Agustiadi Teguh, Akhlus Syafsir, Hidayati Nuning Vita
Center for Maritime Biosciences Studies-Institute for Sciences and Community Service, Jenderal Soedirman University, Kampus Karangwangkal, Jl. dr. Suparno, Purwokerto, 53123, Indonesia.
Marine Science and Fisheries Faculty-Raja Ali Haji Maritime University, Jl. Politeknik Senggarang-Tanjungpinang, Riau Islands Province, 29100, Indonesia.
Heliyon. 2019 May 7;5(5):e01594. doi: 10.1016/j.heliyon.2019.e01594. eCollection 2019 May.
The study aims to isolate the culturable marine bacteria and to assess their potential as the bioremediation agent for petroleum hydrocarbons contamination in marine environment.
Bacteria isolates were obtained by repetitive streaks to obtain purified bacteria on Zobell marine agar plates before further analysis and culture through direct visualization on agar plates. Identification were conducted using 16S rDNA sequence which are compared using NCBI BLAST and, combined with phenotypic and phylogenetic data. The potential use of the selected bacteria was tested by culturing them with two carbon sources i.e., glucose and crude oil.
Fifty-one culturable marine hydrocarbonoclastic bacteria were isolated from the Lombok Strait (LS-3, LS-13, LS-14, LS-15, LS-16 and LS-20) and Indian Ocean (IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25). Twelve isolates were found to degrade crude oil efficiently at a >2% concentration and to grow with crude oil as their sole carbon and energy source. These 12 strains belong to the genus which is well known to produce surface active agents, and the oil displacement assay indicated the production of these agents by these strains. Within the genera , five species (, , , and ) were represented by the 12 identified strains.
Selected strains from the Lombok Strait and Indian Ocean were capable of degrading crude oil (2% v/v) by 43.9-71.9% over 14 days. These results are important for marine bioremediation in Indonesia, which often faces risks of oil spill contamination and disaster.
本研究旨在分离可培养的海洋细菌，并评估其作为海洋环境中石油烃污染生物修复剂的潜力。
通过在佐贝尔海洋琼脂平板上重复划线获得细菌分离株，以获得纯化细菌，然后通过琼脂平板上的直接观察进行进一步分析和培养。使用16S rDNA序列进行鉴定，并与NCBI BLAST进行比较，并结合表型和系统发育数据。通过用两种碳源即葡萄糖和原油培养所选细菌来测试其潜在用途。
从龙目海峡（LS - 3、LS - 13、LS - 14、LS - 15、LS - 16和LS - 20）和印度洋（IO - 1、IO - 6、IO - 8、IO - 19、IO - 24和IO - 25）分离出51株可培养的海洋烃降解细菌。发现12株分离株在浓度>2%时能有效降解原油，并以原油作为唯一碳源和能源生长。这12株菌株属于一个以产生表面活性剂而闻名的属，原油置换试验表明这些菌株产生了这些活性剂。在该属中，12株已鉴定菌株代表了5个种（、、、和）。
从龙目海峡和印度洋中选择的菌株在14天内能够将原油（2% v/v）降解43.9 - 71.9%。这些结果对于经常面临石油泄漏污染和灾难风险的印度尼西亚海洋生物修复具有重要意义。
