Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266072, China.
Mol Biol Rep. 2019 Aug;46(4):4245-4257. doi: 10.1007/s11033-019-04879-4. Epub 2019 May 20.
Sulfide and hypoxia threaten marine organisms in various ways. Anadara broughtonii, a commercial marine bivalve in China which has great potential exposure to sulfide and hypoxia, was selected to test the responses to these stresses. Digital gene expression profile (DGE) analysis was performed on the juveniles' gills after exposed to normal condition (CG group), hypoxia (LO group), and low/high concentration of sulfide (LS/HS group, administered in hypoxia), respectively, using RNA-seq technology. A total of over 30 million clean reads were filtered from each DGE library and over 90% of them were annotated successfully. In total, 774 significant differentially expressed genes (DEGs) were detected and assigned to Gene ontology (GO) classification and KEGG Pathway enrichment analysis. The results show that many of the upregulated DEGs are related to hemoglobin, immunology, and stress responding. In the stressed A. broughtonii, cytochrome P450 and phosphoenolpyruvate carboxykinase may stimulate the glycolysis process to reduce oxygen consumption; Aminoacyl-tRNA synthetases, heat shock protein and protein disulfide isomerase probably help to maintain the genome integrity; Baculoviral IAP repeat-containing protein 2/3, mitogen-activated protein kinase and tumor necrosis factor pathways were probably responsible for protein repair, proteolysis, apoptosis and immune responses to high concentration of sulfide. Combined challenges also induced alternative oxidase and sushi repeat-containing protein, which have indistinct but probably indispensable function in invertebrates. For the first time, comprehensive transcriptome information on A. broughtonii in response to sulfide and hypoxia were provided. Our research offers new insights into the molecular mechanism behind the resistance of shellfish to sulfide and hypoxia.
硫化物和缺氧以各种方式威胁海洋生物。中国的一种商业海洋双壳贝类——文蛤（Anadara broughtonii），具有很大的硫化物和缺氧暴露风险，被选择来测试其对这些压力的反应。使用 RNA-seq 技术，对暴露于正常条件（CG 组）、缺氧（LO 组）和低/高浓度硫化物（LS/HS 组，在缺氧条件下给药）下的幼贝鳃进行了数字基因表达谱（DGE）分析。从每个 DGE 文库中过滤出超过 3000 万个清洁读数，其中超过 90%被成功注释。总共检测到 774 个显著差异表达基因（DEGs），并分配到基因本体（GO）分类和 KEGG 途径富集分析。结果表明，许多上调的 DEGs 与血红蛋白、免疫学和应激反应有关。在受到胁迫的文蛤中，细胞色素 P450 和磷酸烯醇丙酮酸羧激酶可能会刺激糖酵解过程以减少氧气消耗；氨酰-tRNA 合成酶、热休克蛋白和蛋白质二硫键异构酶可能有助于维持基因组完整性；杆状病毒 IAP 重复蛋白 2/3、丝裂原活化蛋白激酶和肿瘤坏死因子途径可能负责蛋白质修复、蛋白酶解、凋亡和对高浓度硫化物的免疫反应。联合胁迫还诱导了交替氧化酶和 sushi 重复蛋白，它们在无脊椎动物中具有不明显但可能必不可少的功能。这是首次提供文蛤对硫化物和缺氧反应的综合转录组信息。我们的研究为贝类对硫化物和缺氧的抗性背后的分子机制提供了新的见解。
