Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Industrial Biotechnology Research Centre, SIRIM Berhad, 1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40911, Shah Alam, Malaysia.
Appl Biochem Biotechnol. 2019 Oct;189(2):690-708. doi: 10.1007/s12010-019-03042-w. Epub 2019 May 21.
The present studies are to evaluate the ability of PB to induce weight loss and urine metabolite profile of Piper betle L. (PB) leaf extracts using metabolomics approach. Dried PB leaves were extracted with ethanol 70% and the studies were performed in different groups of rats fed with high fat (HFD) and normal diet (ND). Then, fed with the PB extract with 100, 300, and 500 mg/kg and two negative control groups given water (WTR). The body weights were monitored and evaluated. Urine was collected and H NMR-based metabolomics approach was used to detect the metabolite changes. Results showed that PB-treated group demonstrated inhibition of body weight gain. The trajectory of urine metabolites showed that PB-treated group gave the different distribution from week 12 to 16 compared with the control groups. In H NMR metabolomic approach analysis, the urine metabolites gave the best separation in principle component 1 and 3, with 40.0% and 9.56% of the total variation. Shared and unique structures (SUS) plot model showed that higher concentration PB-treated group was characterized by high level of indole-3-acetate, aspartate, methanol, histidine, and creatine, thus caused an increased the metabolic function and maintaining the body weight of the animals treated.
本研究旨在利用代谢组学方法评估胡椒（Piper betle L.）叶提取物 PB 诱导体重减轻和尿液代谢物谱的能力。将干燥的 PB 叶用 70%乙醇提取，并在给予高脂肪饮食（HFD）和正常饮食（ND）的不同大鼠组中进行研究。然后，给予 100、300 和 500mg/kg 的 PB 提取物和两个阴性对照组水（WTR）。监测和评估体重。收集尿液并使用基于 1 H NMR 的代谢组学方法检测代谢物变化。结果表明，PB 处理组表现出体重增加的抑制作用。尿液代谢物的轨迹表明，与对照组相比，PB 处理组在第 12 至 16 周的分布不同。在 1 H NMR 代谢组学分析中，尿液代谢物在主成分 1 和 3 中给出了最佳分离，总方差分别为 40.0%和 9.56%。共享和独特结构（SUS）图模型表明，较高浓度的 PB 处理组的特征是高水平的吲哚-3-乙酸、天冬氨酸、甲醇、组氨酸和肌酸，从而导致代谢功能增强并维持动物的体重。
