温度诱导氧化胁迫条件下新分离的微藻钝顶螺旋藻的生物燃料潜力。
Biofuel potential of the newly isolated microalgae Acutodesmus dimorphus under temperature induced oxidative stress conditions.
机构信息
Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Discipline of Wasteland Research, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
出版信息
Bioresour Technol. 2015 Mar;180:162-71. doi: 10.1016/j.biortech.2014.12.102. Epub 2015 Jan 6.
Lack of control over temperature is one of the major issues in large scale cultivation of microalgae. Therefore, it is important to evaluate the effects of cultivation temperature on the growth and physiology of microalgae. In the present study, freshwater microalgae Acutodesmus dimorphus was grown at different temperature in continuous and two stage cultivation. Results revealed that during continuous cultivation A. dimorphus grows better at 35°C than at 25°C and 38°C. At 35°C, A. dimorphus produced 22.7% lipid (containing 59% neutral lipid) and 33.7% carbohydrate along with 68% increase in biomass productivity (23.53mg/L/day) compared to 25°C grown culture. Stress biomarkers like reactive oxygen species, antioxidant enzymes like catalase and ascorbate peroxidase and lipid peroxidation were also lowest in 35°C grown culture which reveals that A. dimorphus is well acclimatized at 35°C.
对温度的控制不足是大规模培养微藻的主要问题之一。因此，评估培养温度对微藻生长和生理的影响非常重要。在本研究中，在连续和两阶段培养中，将淡水微藻斜生栅藻在不同温度下进行培养。结果表明，在连续培养中，斜生栅藻在 35°C 下的生长状况优于 25°C 和 38°C。在 35°C 下，与 25°C 下生长的培养物相比，斜生栅藻产生了 22.7%的脂质（含有 59%的中性脂质）和 33.7%的碳水化合物，生物量生产率增加了 68%（23.53mg/L/天）。在 35°C 下生长的培养物中的应激生物标志物（如活性氧）、抗氧化酶（如过氧化氢酶和抗坏血酸过氧化物酶）和脂质过氧化作用也最低，这表明斜生栅藻在 35°C 下很好地适应了环境。
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