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头部赤霉病的田间病原体组学揭示了由于宿主抗性导致的病原体转录组差异。

Field pathogenomics of head blight reveals pathogen transcriptome differences due to host resistance.

机构信息

a Department of Crop Sciences, University of Illinois at Urbana-Champaign , 1102 S. Goodwin Avenue, Urbana , Illinois 61801.

b High-Performance Biological Computing and the Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign , 1206 W. Gregory Drive, Urbana , Illinois 61801.

出版信息

Mycologia. 2019 Jul-Aug;111(4):563-573. doi: 10.1080/00275514.2019.1607135. Epub 2019 May 21.

DOI:10.1080/00275514.2019.1607135
PMID:31112486
Abstract

head blight (FHB), caused by and other species, is a detrimental disease that affects small grains such as wheat around the world. Management of FHB is difficult, and surveillance as well as a better understanding of pathogen aggressiveness is needed for improved control. disease severity varies depending on the resistance of the host genotype. In this study, we used the field pathogenomics method to investigate gene expression and population structure of isolates collected from wheat lines of varying resistance levels (susceptible, intermediate, and resistant) as well as an axenic control. Differential gene expression was found among isolates collected from different host genotypes. Candidate gene sets were identified for both infection of specific host genotypes and general infection to wheat. Population structure of isolates from different resistance level sources was the same, with all isolates belonging to the NA1 population.

摘要

小麦赤霉病(FHB)由 和其他 种真菌引起,是一种世界性的危害小麦等小粒谷物的病害。防治小麦赤霉病的难度较大,需要加强监测,更好地了解病原菌的侵袭力,以提高防治效果。病害严重度取决于宿主基因型的抗性。本研究采用田间病原组学方法,调查了从小麦不同抗性(感病、中抗和高抗)品系以及无菌对照中采集的分离株的基因表达和种群结构。从小麦不同抗性基因型中采集的分离株的基因表达存在差异。鉴定了与特定宿主基因型的 侵染和对小麦的一般侵染相关的候选基因集。不同抗性来源的分离株的种群结构相同,所有分离株均属于 NA1 种群。

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