Adhikari Pragya, Mideros Santiago X, Jamann Tiffany M
Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Front Plant Sci. 2021 May 25;12:675208. doi: 10.3389/fpls.2021.675208. eCollection 2021.
Pathogens that infect more than one host offer an opportunity to study how resistance mechanisms have evolved across different species. infects both maize and sorghum and the isolates are host-specific, offering a unique system to examine both compatible and incompatible interactions. We conducted transcriptional analysis of maize and sorghum in response to maize-specific and sorghum-specific isolates and identified functionally related co-expressed modules. Maize had a more robust transcriptional response than sorghum. responsive genes were enriched in core orthologs in both crops, but only up to 16% of core orthologs showed conserved expression patterns. Most changes in gene expression for the core orthologs, including hub genes, were lineage specific, suggesting a role for regulatory divergent evolution. We identified several defense-related shared differentially expressed (DE) orthologs with conserved expression patterns between the two crops, suggesting a role for parallel evolution of those genes in both crops. Many of the differentially expressed genes (DEGs) during the incompatible interaction were related to quantitative disease resistance (QDR). This work offers insights into how different hosts with relatively recent divergence interact with a common pathogen. Our results are important for developing resistance to this critical pathogen and understanding the evolution of host-pathogen interactions.
感染多种宿主的病原体为研究抗性机制如何在不同物种间进化提供了契机。[病原体名称]同时感染玉米和高粱，且分离株具有宿主特异性，这为研究亲和与非亲和互作提供了独特的系统。我们对玉米和高粱针对玉米特异性和高粱特异性分离株的反应进行了转录分析，并鉴定了功能相关的共表达模块。玉米的转录反应比高粱更强烈。响应基因在两种作物的核心直系同源基因中富集，但只有高达16%的核心直系同源基因表现出保守的表达模式。核心直系同源基因（包括枢纽基因）的大多数基因表达变化具有谱系特异性，这表明调控趋异进化发挥了作用。我们鉴定了几种与防御相关的共享差异表达（DE）直系同源基因，它们在两种作物之间具有保守的表达模式，这表明这些基因在两种作物中平行进化发挥了作用。非亲和互作过程中的许多差异表达基因（DEG）与数量抗病性（QDR）相关。这项工作为了解亲缘关系较近的不同宿主如何与共同病原体相互作用提供了见解。我们的结果对于培育对这种关键病原体的抗性以及理解宿主 - 病原体相互作用的进化具有重要意义。
