Ohtake H, Cervantes C, Silver S
J Bacteriol. 1987 Aug;169(8):3853-6. doi: 10.1128/jb.169.8.3853-3856.1987.
CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more resistant to CrO4(2-) than those grown with 0.15 mM djenkolic acid, which derepressed the 35SrO4(2-) uptake system. Kinetics of 51CrO4(2-) uptake by P. fluorescens with and without the plasmid showed that the Vmax for 51CrO4(2-) uptake with the resistant strain was 2.2 times less than the Vmax for the sensitive strain, whereas the Km remained constant.
荧光假单胞菌LB300(pLHB1)对CrO4(2-)的抗性与相对于无质粒菌株LB303而言CrO4(2-)摄取减少有关。51CrO4(2-)主要通过SO4(2-)主动转运系统进行转运;因此,用0.15 mM半胱氨酸(SO4(2-)转运系统的一种阻遏物)培养的细胞比用0.15 mM 豆科氨酸(它可解除35SrO4(2-)摄取系统的阻遏)培养的细胞对CrO4(2-)的抗性要强得多。有质粒和无质粒的荧光假单胞菌对51CrO4(2-)的摄取动力学表明,抗性菌株对51CrO4(2-)的摄取Vmax比敏感菌株的Vmax低2.2倍,而Km保持不变。