Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
National Research Centre (NRC), Medical Physiology Department, Egypt.
Eur Cytokine Netw. 2019 Mar 1;30(1):15-22. doi: 10.1684/ecn.2019.0421.
There are a limited number of studies that report the polarization of the immune system toward the production of T helper 1 (Th1), Th2, or Th17-type cytokines in patients with Behçet's disease (BD). Here, we aimed to detect the presence of various cytokines in serum samples of Egyptian BD patients and to determine the correlation between their production levels and clinical manifestations. To that aim, serum levels of IFN-γ, IL-10, IL-6, and IL-17 measured by ELISA were determined in BD patients with active or inactive disease to evaluate their clinical relevance. The results of the present study show significantly elevated levels of IL-17 and IL-6, as well as a reduction in IL-10, and no change in IFN-γ, in sera of BD patients, as compared to the healthy control group. Moreover, IL-6 serum levels were increased in BD patients in active stages of disease and correlated with arthritic manifestations. On the other hand, IL-10 serum levels were significantly decreased in patients with gastrointestinal tract complications. Furthermore, a positive correlation was observed between IL-10 serum levels and ocular manifestations in BD patients, in contrast to those of IL-17, showing no correlation with the different clinical manifestations. Taken together, the magnitude of IL-6 serum levels could be a potential marker for arthritic manifestations and disease activity, whereas those of IFN-γ, IL-10, and IL-17 cannot be considered predictors for different clinical manifestations in patients with BD.
目前仅有少数研究报告了贝切特病（BD）患者的免疫系统向产生辅助性 T 细胞 1（Th1）、Th2 或 Th17 型细胞因子的极化。在这里，我们旨在检测埃及 BD 患者血清样本中各种细胞因子的存在，并确定其产生水平与临床表现之间的相关性。为此，通过 ELISA 测定 BD 患者活动期和非活动期血清中 IFN-γ、IL-10、IL-6 和 IL-17 的水平，以评估其临床相关性。本研究结果表明，与健康对照组相比，BD 患者血清中 IL-17 和 IL-6 水平显著升高，IL-10 水平降低，IFN-γ 水平无变化。此外，BD 患者活动期关节炎表现时血清中 IL-6 水平升高。另一方面，胃肠道并发症患者的血清 IL-10 水平显著降低。此外，BD 患者中观察到 IL-10 血清水平与眼部表现呈正相关，而与 IL-17 则无相关性。总之，IL-6 血清水平的高低可能是关节炎表现和疾病活动的潜在标志物，而 IFN-γ、IL-10 和 IL-17 不能作为 BD 患者不同临床表现的预测因子。
