Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Carbohydr Polym. 2019 May 1;211:217-226. doi: 10.1016/j.carbpol.2019.01.102. Epub 2019 Jan 31.
Alhagi honey polysaccharides (AHP) have been widely studied as immunomodulators. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles have been frequently used to control the release of drugs. In this study, AHP was extracted and encapsulated within PLGA (AHPP). Enhancement of immune activity in vitro and the adjuvanticity when inoculated with OVA were evaluated. The results demonstrated that the average molecular weight of AHP was 46.8 kDa and possessed typical polysaccharide absorption peaks. The entrapment efficiency for AHP within AHPP was 65.76 ± 3.31%. AHPP significantly stimulated phagocytic activity, MHCII and CD86 expression in macrophages. Further investigation showed that AHPP/OVA significantly enhanced lymphocyte proliferation and improved the CD4/CD8 T cell ratio. Moreover, AHPP/OVA treatment significantly increased IgG levels and up-regulated Th-associated cytokines with overall Th1 polarization. These studies demonstrated that AHP encapsulated within PLGA as a vaccine delivery system enhanced adaptive immunity.
麻糖复合多糖（AHP）已被广泛研究为免疫调节剂。聚乳酸-羟基乙酸共聚物（PLGA）纳米粒常用于控制药物释放。在本研究中，AHP 被提取并封装在 PLGA 中（AHPP）。评估了体外免疫活性增强作用和与 OVA 接种时的佐剂作用。结果表明，AHP 的平均分子量为 46.8 kDa，具有典型的多糖吸收峰。AHPP 中 AHP 的包封效率为 65.76±3.31%。AHPP 显著刺激了巨噬细胞的吞噬活性、MHCII 和 CD86 的表达。进一步的研究表明，AHPP/OVA 显著增强了淋巴细胞增殖，并改善了 CD4/CD8 T 细胞比例。此外，AHPP/OVA 处理显著增加了 IgG 水平，并上调了 Th 相关细胞因子，总体上呈 Th1 极化。这些研究表明，PLGA 包封的 AHP 作为疫苗传递系统增强了适应性免疫。
