伊朗东北部霍拉桑省人类分离株的基因分型
Genotyping of Isolates from Human in Khorasan Province, North-Eastern Iran.
作者信息
Berenji Fariba, Shamsian Seyed Aliakbar, Nouri Daloee Marziyeh, Fattahi Masoom Seyed Hossein, Moghaddas Elham
机构信息
Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Thoracic Surgery, Cardiothoracic Surgery and Transplant Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Parasitol. 2019 Jan-Mar;14(1):52-58.
BACKGROUND
Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of isolates collected from human surgically.
METHODS
Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 () and NADH dehydrogenase subunit I ().
RESULTS
Overall, 55 out of 60 cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified.
CONCLUSION
Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.
背景
人体包虫病在伊朗东北部呈地方流行。本研究旨在调查从接受手术的患者中收集的分离株的分子多样性。
方法
2015年至2016年期间，从马什哈德医科大学的加姆医院和伊玛目礼萨医院通过手术收集了60个人体包虫囊肿（58个肺囊肿和2个肝囊肿）。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析内部转录间隔区1（ITS1）基因，并对编码线粒体细胞色素c氧化酶亚基1（COX1）和NADH脱氢酶亚基I（ND1）的基因片段进行测序，对囊肿进行特征分析。
结果
总体而言，60个囊肿中有55个（91.6%）被确定为G1株，4例（6.6%）被确定为G6株，1个样本未被鉴定。
结论
尽管大霍拉桑地区人体患者中羊株（G1）占主导地位，但该地区应注意预防骆驼-犬循环。
Zotero 插件