Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Acta Parasitol. 2021 Jun;66(2):446-454. doi: 10.1007/s11686-020-00293-0. Epub 2020 Oct 20.
Identification of different genotypes of echinococcal cyst in various domestic herbivores and humans within the target area was the principal aim of the present study, performed using sequence data of cox1 and nad1 mitochondrial genes.
A total of 57 cystic echinococcosis (CE) cysts were isolated from indigenous livestock including 45 cattle, 9 sheep and 3 goats from several slaughterhouses in Guilan Province. Moreover, 12 formalin-fixed paraffin-embedded (FFPE) CE cyst tissues from humans were also included, obtained from the archives of several hospitals in Rasht, the capital of Guilan. Genetic sequencing was conducted using mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes.
Our results found that E. granulosus sensu stricto (s.s.) and E. ortleppi were present in 92.7% and 7.2% isolates, respectively. E. granulosus s.s. (genotypes G1 and G3) and E. ortleppi were isolated from various livestock whereas all CE cysts isolated from humans were E. granulosus s.s. G1 genotype.
We found that E. granulosus s.s. G1 was the predominant genotype within the study region. This is the first study to report E. ortleppi in cattle in Iran.
本研究旨在利用 cox1 和 nad1 线粒体基因序列数据，鉴定目标区域内不同家畜和人类的细粒棘球蚴（CE）囊的不同基因型。
从伊朗吉兰省多个屠宰场的 45 头牛、9 只绵羊和 3 只山羊等本地家畜中分离出 57 个囊性包虫病（CE）囊肿，此外，还从拉什特（吉兰省省会）的几家医院档案中获得了 12 个福尔马林固定石蜡包埋（FFPE）CE 囊肿组织。使用线粒体细胞色素 c 氧化酶亚单位 1（cox1）和 NADH 脱氢酶亚单位 1（nad1）基因进行遗传测序。
我们的结果发现，92.7%的分离株为细粒棘球蚴亚种（s.s.），7.2%为 E. ortleppi。细粒棘球蚴亚种（G1 和 G3 基因型）和 E. ortleppi 从各种家畜中分离出来，而从人类中分离出来的所有 CE 囊肿均为细粒棘球蚴亚种 G1 基因型。
我们发现，研究区域内 G1 基因型是主要的细粒棘球蚴亚种。这是首次在伊朗报告牛感染 E. ortleppi 的研究。
