Nikmanesh Bahram, Mirhendi Hossein, Ghalavand Zohreh, Alebouyeh Masoud, Sharbatkhori Mitra, Kia EshratBeigom, Mohebali Mehdi, Eghbali Maryam, Rokni Mohammad Bagher
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iranian Tissue Bank & Research Center, Tehran University of Medical Science, Tehran, Iran.
Iran J Parasitol. 2014 Mar;9(1):20-7.
The present study was aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in Iran.
Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected to morphological microscopic examinations. Protoscoleces were removed from each cyst and their total genomic DNAs were extracted. PCR was performed to amplify fragments of 450 and 400 base pair (bp) for cox1 and nad1 genes, respectively. Genotype diversity and sequence variation of the strains were studied by bioinformatics software and in comparison with those mtDNA sequences already deposited in GenBank.
Sixteen, (53.3%), 13 (43.3%), and 1 (3.3%) samples were related to lung, liver, and spleen, respectively. The remained 17 unfertile samples were excluded from the study. From the 29 isolates, 86.7% (n=26) and 10% (n=3) were related to G1, and G3 genotypes, respectively. The sole isolate with G6 genotype was obtained from lung sample. Analysis of concatenated sequences of cox1+nad1 indicated the presence of 11 haplotypes among our strains that were related to genotypes G1 (n=9), G3 (n=1) and G6 (n=1).
In consistent to other reports from Iran, genotypes G1, G3, and G6 were observed in our human isolates. The rate of G3 genotype was however higher than other studies implying that human can be considered as a new appropriate host for G3 genotype. Further studies with more sample size from different geographic areas of Iran are needed for E. granulosus mapping.
本研究旨在利用伊朗人临床样本中收集的细粒棘球绦虫分离株的两个线粒体基因cox1和nad1，研究其分子多样性。
2010年至2012年期间，从德黑兰的两家医院通过手术收集了47例人包虫囊肿。为了确定原头蚴的生育力，对囊液进行形态学显微镜检查。从每个囊肿中取出原头蚴并提取其总基因组DNA。进行PCR分别扩增cox1和nad1基因的450和400碱基对（bp）片段。通过生物信息学软件研究菌株的基因型多样性和序列变异，并与GenBank中已存的那些线粒体DNA序列进行比较。
16例（53.3%）、13例（43.3%）和1例（3.3%）样本分别与肺、肝和脾有关。其余17例不育样本被排除在研究之外。在29个分离株中，86.7%（n = 26）和10%（n = 3）分别与G1和G3基因型有关。唯一的G6基因型分离株来自肺样本。cox1 + nad1串联序列分析表明，我们的菌株中有11种单倍型，分别与G1基因型（n = 9）、G3基因型（n = 1）和G6基因型（n = 1）有关。
与伊朗的其他报告一致，在我们的人类分离株中观察到了G1、G3和G6基因型。然而，G3基因型的比例高于其他研究，这意味着人类可被视为G3基因型的新的合适宿主。需要对来自伊朗不同地理区域的更多样本进行进一步研究，以绘制细粒棘球绦虫图谱。
